| 反硝化技术对模拟养殖池塘修复的研究 |
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| 引用本文： | 尹艳娥,沈新强,晁敏,全为民,王云龙.反硝化技术对模拟养殖池塘修复的研究[J].农业环境保护,2009(8):1727-1732. |
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| 作者姓名： | 尹艳娥 沈新强 晁敏 全为民 王云龙 |
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| 作者单位： | 农业部海洋与河口渔业重点开放实验室,中国水产科学研究院东海水产研究所,上海200090 |
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| 基金项目： | 中央级公益性科研院所基本科研业务费专项资金（中国水产科学研究院东海水产研究所）资助项目（2007T01） |
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| 摘 要： | 浙江奉化市池塘的底泥经过反复培养、驯化，从中筛选、分离出反硝化细菌，在模拟实验条件下，研究其对不同浓度的硝酸盐氮和亚硝酸盐氮的去除情况，讨论反硝化菌种的生长情况。结果表明，在初始浓度为1、25、50mg·L^-1的硝酸盐氮和亚硝酸盐氮模拟池塘中，随着实验的进行，对污染物的去除效果逐渐提高。其中在1mg·L^-1的浓度组中，3d内硝酸盐氮和亚硝酸盐氮去除率就分别达到了95．8％和90．2％；在25mg·L^-1的浓度组中，第6d硝酸盐氮和亚硝酸盐的去除率分别为93．8％和87．8％；在50mg·L^-1的浓度组中，第6d硝酸盐氮和亚硝酸盐的去除率分别为89．7％和78．7％。此外，反硝化菌对硝酸盐氮的去除效果略优于亚硝酸盐氮，而且硝酸盐氮和亚硝酸盐氮的浓度越低，对其去除效果越好，达到稳定状态的时间越短。在模拟池塘中，菌种的生长情况与硝酸盐氮和亚硝酸盐氮的浓度呈负相关，即污染物的浓度越高反硝化菌的生长情况越差。对反硝化菌的生态影响因子研究表明，其反硝化最适宜的pH值为6～7，温度为25～35℃；而且在同一pH值和温度条件下，硝酸盐氮和亚硝酸盐氮浓度越低，反硝化菌对其去除效果越好。
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| 关 键 词： | 反硝化菌 模拟养殖池塘 生物修复 硝酸盐氮 亚硝酸盐氮 |
Investigation on Remediation of Simulant Aquaculture Pond by Denitrification Technology |
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| YIN Yan-e,SHEN Xin-qiang,CHAO Min,QUAN Wei-min,WANG Yun-long.Investigation on Remediation of Simulant Aquaculture Pond by Denitrification Technology[J].Agro-Environmental Protection,2009(8):1727-1732. |
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| Authors: | YIN Yan-e SHEN Xin-qiang CHAO Min QUAN Wei-min WANG Yun-long |
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| Institution: | (East China Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Key and Open Laboratory of Marine and Estuarine Fishery, Ministry of Agriculture, Shanghai 200090, China) |
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| Abstract: | Denitrifying bacteria was screened from repeatedly cultivating sediment of aquacuhure pond in Fenghua, Zhejiang Province. The function of denitrifying bacteria was analyzed according to the quality indexes, such as removal efficiency of nitrate nitrogen（ NO3^- -N ）, nitrite nitrogen （ NO2^- -N ） and growth of bacteria in the simulated condition. The results indicated that the denitrifying bacteria could rapidly improve the water environment and increased the removal efficiency of NO3^- -N and NO2^- -N. When the initial concentration of NO3^- -N and NO2^- -N were respectively 1, 25 and 50 mg·L^-1 in the simulated pond, removal efficiency of pollutants gradually increased with increasing time. In three days, the removal efficiency of NO3^- -N and NO2^- -N reached 95.8% and 90.2% when the initial concentration of NO3^- -N and NO2^- -N were 1 mg·L^-1. On the sixth day, 93.8% and 87.8% of NO3^- -N and NO2^- -N were removed by ddnitrifying bacteria when the initial concentration of NO3^- -N and NO2^- -N were 25 mg·L^-1. Approximately 89.7% and 78.7% were removed when the initial concentration of NO3^- -N and NO2^- -N were 50 mg.L^-1.And the removal efficiency and steady efficiency increased with decreasing concentration of NO3^- -N and NO2^- -N. And the correlation between the concentration of NO3^- -N, NO2^- -N and the numbers of denitrifying bacteria was researched. The concentration of NO3^- -N and NO2^- -N were higher, the numbers of denitrifying bacteria were less. The factors affecting denitrifying bacteria were also discussed. The results indicated that the most suitable pH value and temperature for denitrification were 6-7 and 25-35 ℃, respectively. And in the same condition for pH value and temperature, the concentration of NO3^- -N and NO2^- -N were higher, the removal efficiency were better. |
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| Keywords: | denitrifying bacteria simulated aquaculture pond bioremediation nitrate nitrogen nitrite nitrogen |
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