| 微生物絮凝剂产生菌的筛选及其絮凝活性研究 |
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| 引用本文： | 江慧华,马森,方继敏.微生物絮凝剂产生菌的筛选及其絮凝活性研究[J].安徽农业科学,2011,39(15):8990-8993. |
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| 作者姓名： | 江慧华 马森 方继敏 |
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| 作者单位： | 武夷学院环境与建筑工程系;武汉理工大学资源与环境工程学院; |
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| 基金项目： | 武夷学院青年教师学术研究支持计划项目(XQL05025); 福建省教育厅B类科研项目(JB05191) |
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| 摘 要： | 目的]筛选出具有高絮凝活性的微生物产生菌,并观察研究微生物产生菌的外观形貌,并确定微生物产生菌的最佳培养条件。方法]分别从土壤、活性污泥、食品厂污水和垃圾渗滤液中筛选出具有絮凝活性的菌株若干,然后在不同的培养基条件下通过富集培养、平板分离、纯化培养和复筛,筛选出具有高絮凝活性的菌种。在显微镜下观察菌种的形貌。最后考察了培养温度、培养时间和摇床转速对絮凝效果的影响,对培养条件进行了优化。结果]从土壤、活性污泥、食品厂污水和垃圾渗滤液中筛选出具有絮凝活性的菌种8株,然后在不同的培养基条件下通过富集培养、平板分离、纯化培养和复筛等,筛选出絮凝活性超过90%菌种2株,在显微镜下初步鉴定为杆菌。以絮凝活性最高的F-4为例,该菌产絮凝剂的最佳培养条件为：培养时间56 h、培养温度30℃、摇床转速150 r/min。结论]在该条件下,F-4菌株对高岭土悬浊液的絮凝率达到93.1%。
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| 关 键 词： | 微生物絮凝剂 菌种筛选 絮凝率 培养条件 |
Screening of Microbial Flocculant Producing Strain and Investigation of Its Flocculating Activity |
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| JIANG Hui-hua et al.Screening of Microbial Flocculant Producing Strain and Investigation of Its Flocculating Activity[J].Journal of Anhui Agricultural Sciences,2011,39(15):8990-8993. |
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| Authors: | JIANG Hui-hua |
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| Institution: | JIANG Hui-hua et al(Department of Environmental and Civil Engineering,Wuyi University,Wuyishan,Fujian 354300) |
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| Abstract: | Objective]The research aimed to screen microbial flocculant-producing strain of high flocculating activity,the morphology of flocculant-producing strain and the optimum culture condition were studied.Method]Some strains of flocculant-producing microorganism with flocculating activity were screened from soil,activated sludge,food factory wastewater and landfill leachate.Some of strains of flocculant-producing microorganism with high-flocculating activity were isolated by enrichment culture,plate method,pur... |
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| Keywords: | Microbial flocculant Strain screening Flocculation rate Culture condition |
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