| HPLC谱法测定蒲公英不同器官中绿原酸的含量 |
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| 引用本文： | 陈萍,王建刚.HPLC谱法测定蒲公英不同器官中绿原酸的含量[J].安徽农业科学,2011,39(16):9600-9601. |
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| 作者姓名： | 陈萍 王建刚 |
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| 作者单位： | 1. 吉林化工学院化学与制约工程学院,吉林,吉林,132022
2. 吉林化工学院分析测试中心,吉林,吉林,132022 |
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| 摘 要： | 目的]建立HPLC法测定蒲公英不同器官中绿原酸含量的方法。方法]采用Shim-pack VP-ODS（4.6 mm×150.00 mm,5μm）色谱柱;以甲醇-0.4%磷酸水溶液为流动相进行梯度洗脱;流速为1.0 ml/min,检测波长为326 nm;柱温为40℃;进样量为20μl。结果]绿原酸的线性范围为0.791 20~79.120μg/ml,（r=0.999 8）,回收率为98.00%~101.5%。蒲公英叶、根、花和花茎中绿原酸含量分别为1.212、0.536、0.448、0.417 mg/g。结论]该研究为蒲公英中绿原酸含量测定提供了一种快速、准确的方法;蒲公英叶中绿原酸含量最高,这为更好地开发利用蒲公英这一药食植物资源提供了依据。
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| 关 键 词： | 蒲公英 不同器官 绿原酸 HPLC |
Determination of Chlorogenic Acid of Different Organs from Taraxacum mongolicum by High Performance Liquid Chromatography |
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| CHEN Ping et al.Determination of Chlorogenic Acid of Different Organs from Taraxacum mongolicum by High Performance Liquid Chromatography[J].Journal of Anhui Agricultural Sciences,2011,39(16):9600-9601. |
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| Authors: | CHEN Ping |
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| Institution: | CHEN Ping et al(College of Chemistry and Pharmaceutical Engineering,Jilin Institute of Technology,Jilin,Jilin 132022) |
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| Abstract: | Objective] To establish a method for determining contents of chlorogenic acid in different organs from Taraxacum mongolicum by HPLC.Method] The HPLC was carried out with Shim-pack VP-ODS column(4.6 mm×150.0 mm,5 μm) using mixture of methanol and 0.4 % phosphoric acid solution in different ratio as mobile phase.Other conditions were: flow rate of 1.0 ml/min,detection wavelength of 326 nm,column temperature of 40 ℃ and sampling amount of 20 μl.Result] When the concentration of chlorogenic acid ranged from 0.791 2 to 79.120 0 μg/ml,the linear relationship between concentration and peak area was good(r=0.999 8).The recovery was 98.00%-101.50%.Leaves、roots、flowers and scapes of Taraxacum mongolicum were 1.212,0.536,0.448 and 0.417 mg/g in chlorogenic acid,respectively.Conclusion] The method established in this work was a rapid and accurate method for determining chlorogenic acid from Taraxacum mongolicum.Leaf of Taraxacum mongolicum was highest in chlorogenic acid,which provided basis for better developing and applying of Taraxacum mongolicum. |
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| Keywords: | Taraxacum mongolicum Different organs Chlorogenic acid HPLC |
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