| 小麦幼苗根系在Cr6+胁迫下生长和DNA含量的变化 |
| |
| 引用本文： | 黑淑梅.小麦幼苗根系在Cr6+胁迫下生长和DNA含量的变化[J].安徽农业科学,2011,39(14):8261-8262,8301. |
| |
| 作者姓名： | 黑淑梅 |
| |
| 作者单位： | 延安大学生命科学学院,陕西延安,716000 |
| |
| 摘 要： | 目的]研究重金属Cr6＋对小麦幼苗根系生长和DNA含量的影响。方法]用浓度5100 mg/L Cr6＋分别处理3和10 d龄小麦幼苗,测定生长指标,采用紫外分光光度法和电泳法测定DNA含量变化。结果]除了Cr6＋〉20 mg/L时,3d龄幼苗的根数小于对照根数外,其余处理根数均增加;而根长、根系鲜重和干重、DNA含量均随着Cr6＋浓度的增加而降低。3d龄幼苗比10 d龄幼苗对Cr6＋更敏感。琼脂糖凝胶电泳显示,对照根尖DNA带基本完整,而Cr6＋处理的DNA则发生了降解,出现拖尾现象。结论]Cr6＋处理影响了小麦幼苗根系的正常生长并导致DNA含量下降。
|
| 关 键 词： | Cr6+ 小麦幼苗 根系 DNA含量 |
Research on the Root Grown of Wheat Seedling under Stress of Cr6+ and the Variation in Content of DNA |
| |
| HEI Shu-mei.Research on the Root Grown of Wheat Seedling under Stress of Cr6+ and the Variation in Content of DNA[J].Journal of Anhui Agricultural Sciences,2011,39(14):8261-8262,8301. |
| |
| Authors: | HEI Shu-mei |
| |
| Institution: | HEI Shu-mei(College of Life Sciences,Yanan University,Yanan,Shaanxi 716000) |
| |
| Abstract: | Objective] The effect of heavy metal-Cr6+ on the root growth of wheat seedling and the content of DNA.was analyzed.Method] The growth index of 3——10 day-old wheat seedling,treated with Cr6+ with the concentration of 5-100 mg/L,respectively,was measured,and content of DNA was tested with the method of UV spectrophotometry and electrophoresis.Result] Except the treatment of Cr6+ > 20 mg/L,in which,the number of root in 3 day-old wheat seedling was less than that of the CK,the number of root in other treatments was increased;and the length fresh weight and dry weight of root,the content of DNA were increased with the concentration-increasing of Cr6+.3 day-old seedling was more sensitive to Cr6+ than 10 day-old seedling.The analysis of agarose gel electrophoresis indicated that the DNA in root tip of CK was basic integrity and it was degraded in all of treatments',showing its smearing.Conclusion] The treatment of Cr6+ affected the normal root growth of wheat seedling and led the content-decreasing of DNA. |
| |
| Keywords: | Cr6 Wheat seedling Root Content of DNA |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
