| 菜芙蓉醇提物金丝桃苷含量测定及对肿瘤细胞生长抑制作用 |
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| 引用本文： | 仇燕,庞丽然,李志伟,宋建军,李振侠.菜芙蓉醇提物金丝桃苷含量测定及对肿瘤细胞生长抑制作用[J].安徽农业科学,2011,39(14):8331-8333. |
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| 作者姓名： | 仇燕 庞丽然 李志伟 宋建军 李振侠 |
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| 作者单位： | 河北科技大学生物科学与工程学院,河北石家庄,050018;河北科技大学化学与制药工程学院,河北石家庄,050018 |
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| 基金项目： | 河北科技大学大学生科技创新基金项目 |
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| 摘 要： | 目的]建立测定菜芙蓉（Abelmoschus manihot（L.）Medic）醇提物金丝桃苷含量的方法以及研究菜芙蓉醇提物对Hela肿瘤细胞生长的抑制效果。方法]采用体积分数为80%的乙醇提取总黄酮,HPLC法测定菜芙蓉不同植株部位中金丝桃苷含量。色谱柱为伊利特C18（4.6 mm×250 mm,5μm）,流动相为甲醇-0.05%磷酸溶液（V/V,60∶40）,流速为1.0 ml/min,柱温为25℃,检测波长为360 nm和进样量为10μl。另外,MTT法测定菜芙蓉提取物体外抗Hela肿瘤细胞活性。结果]金丝桃苷在0.040.4 mg/ml范围内与峰面积呈良好的线性关系,相关系数为0.999 8。菜芙蓉不同部位金丝桃苷含量分布为：花〉叶〉茎〉根〉籽,花中平均含量最高达4.002%。平均回收率和RSD值分别为99.23%和1.08%（n=6）。菜芙蓉黄酮提取物能够有效地抑制Hela细胞的生长,IC50约为228μg/ml。结论]该方法准确、简便、重复性好,可作为菜芙蓉金丝桃苷定量分析方法;菜芙蓉总黄酮具有抑制肿瘤细胞生长的作用。
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| 关 键 词： | 菜芙蓉(Abelmoschus manihot(L.)Medic) 金丝桃苷 高效液相色谱法 生长抑制 Hela细胞 |
Determination of Hyperoside in Ethanolic Extract of Abelmoschus manihot (L.) Medic and Its Growth Inhibitory Effects on Tumor Cells |
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| QIU Yan et al.Determination of Hyperoside in Ethanolic Extract of Abelmoschus manihot (L.) Medic and Its Growth Inhibitory Effects on Tumor Cells[J].Journal of Anhui Agricultural Sciences,2011,39(14):8331-8333. |
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| Authors: | QIU Yan |
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| Institution: | QIU Yan et al(College of Bioscience & Engineering,Hebei University of Science and Technology,Shijiazhuang,Hebei 050018) |
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| Abstract: | Objective]To establish a method for determining the contents of hyperoside in ethanolic extract of Abelmoschus manihot(L.) Medic,and study its growth inhibitory effects on Hela cells.Method] Using 80% ethanol as solvent for extracting flavonoid,the contents of hyperoside were determined by HPLC.The HPLC conditions were:chromatographic column of Yilite C18(4.6 mm×250 mm,5 μm) column,mobile phase of methanol and 0.05% phosphoric acid solution(V/V,60:40),flow rate of 1.0 ml/min,column temperature of 25 ℃,detection wavelength of 360 nm and sampling volume of 10 μl.Cell growth inhibitory effects on Hela cells were assessed by MTT assay.Results] The hyperoside content presented a good linear relationship with peak area in the range of 0.04 to 0.4 mg/ml with correlation coefficient at 0.999 8.The content of hyperoside decreased in the following order,flower,leaf,stem,root and seed.The average content in flower was the highest that up to 4.002%.The average recovery and RSD were 99.23% and 1.08%(n=6),respectively.Flavonoids extract of Abelmoschus manihot(L.) Medic could effectively suppress the growth of Hela cells,IC50 of which was 228 μg/ml.Conclusion] The method was accurate,simple and reproducible that can be used for determining the hyperoside in Abelmoschus manihotc(L.) Medic.The flavonoids extracts had strong growth inhibitory effects on Hela cells. |
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| Keywords: | Abelmoschus manihot(L ) Medic Hyperoside HPLC Growth inhibition Hela cell |
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