| 吴茱萸HPLC指纹图谱的建立及3种成分含量的测定 |
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| 引用本文： | 曾金祥,刘勇,黄碧涛,杨雅琴,朱继孝,张忠立,罗光明,熊红红.吴茱萸HPLC指纹图谱的建立及3种成分含量的测定[J].安徽农业科学,2011,39(14):8273-8275,8278. |
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| 作者姓名： | 曾金祥 刘勇 黄碧涛 杨雅琴 朱继孝 张忠立 罗光明 熊红红 |
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| 作者单位： | 江西中医学院江西省中药种质资源工程技术研究中心;南昌市洪都中医院; |
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| 基金项目： | 江西中医学院重点学科青年教师培养计划资助项目(2010023) |
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| 摘 要： | 目的]建立吴茱萸（FRUCTUS EVODIAE）药材的指纹图谱,并对吴茱萸药材中绿原酸、金丝桃苷和吴茱萸碱进行同时测定。方法]HPLC条件为：Hypersil C18色谱柱（250.0 mm×4.6 mm,5μm）;流动相为乙腈-1%乙酸水溶液,梯度洗脱;流速为1.0 ml/min;检测波长为270nm;进样量为10μl。结果]不同产地的吴茱萸药材指纹图谱共有19个共有峰,共有峰相对保留时间RSD值小于2.50%,相对峰面积RSD值为2.10%。整体来看,江西栽培的吴茱萸药材中绿原酸、金丝桃苷和吴茱萸碱含量要高于贵州、云南等地吴茱萸药材。结论]该研究首次建立了吴茱萸药材的指纹图谱并同时测定了吴茱萸药材中的绿原酸、金丝桃苷和吴茱萸碱含量,为不同产地、不同采收期吴茱萸药材的质量评价提供了更好的技术手段。
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| 关 键 词： | 吴茱萸（FRUCTUS EVODIAE） 指纹图谱 含量测定 |
Establishment of HPLC Fingerprint of FRUCTUS EVODIAE and Determination of Three Components |
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| ZENG Jin-xiang et al.Establishment of HPLC Fingerprint of FRUCTUS EVODIAE and Determination of Three Components[J].Journal of Anhui Agricultural Sciences,2011,39(14):8273-8275,8278. |
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| Authors: | ZENG Jin-xiang |
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| Institution: | ZENG Jin-xiang et al(The Chinese Medicine Germplasm Resource Engineering Technology Reseach Center of Jiangxi Province,Jiangxi University of Traditional Chinese Medicines,Nanchang,Jiangxi 330004) |
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| Abstract: | Objective] To establish the fingerprint of FRUCTUS EVODIAE and to determine the contents of chlorogenic acid,hyperoside and evodiamine.Method]The chromatographic separation was performed on a C18 column(250.0 mm×4.6 mm,5 μm) with mobile phase of acenitrile-1% acetic acid and flow rate of 1.0 ml/min.The samples all were detected at the wavelength of 270 nm.Result] There were 19 common peaks in the fingerprint of 11 samples with the RSD value of the relative retention time and peak area less than 2.50% and 3.50%,respectively.In general,FRUCTUS EVODIAE made in Jiangxi Province was higher in contents of chlorogenic acid,hyperoside and evodiamine than that produced in Guizhou and Yunnan.Conclusion] The fingerprint and simultaneous determination of chlorogenic acid,hyperoside and evodiamine in FRUCTUS EVODIAE were firstly studied,which provided better means for the quality control of FRUCTUS EVODIAE produced in different places and different time,and provided technique support for GAP cultivation of FRUCTUS EVODIAE. |
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| Keywords: | FRUCTUS EVODIAE Fingerprint Content determination |
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