| 水稻CBB30Ⅰ抗白叶枯病相关基因的差异表达分析 |
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| 引用本文： | 董旭,张晓平,赵开军,王春连.水稻CBB30Ⅰ抗白叶枯病相关基因的差异表达分析[J].作物杂志,2012(4):20-25. |
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| 作者姓名： | 董旭 张晓平 赵开军 王春连 |
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| 作者单位： | 中国农业科学院作物科学研究所 |
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| 基金项目： | 国家自然科学基金项目(31171812) |
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| 摘 要： | 水稻白叶枯病是水稻生产中最严重病害之一。前期鉴定发掘的水稻资源材料Y238对白叶枯病具有广谱抗性,其衍生的以IR24为遗传背景的基因导入系CBB30Ⅰ保持了广谱抗性。利用抑制消减杂交(suppression subtractive hybridization,SSH)技术构建CBB30Ⅰ与感病品种IR24间的差异表达cD-NA文库,RT-PCR分析抗病相关基因表达情况。经反向Northern blot杂交检测、测序和GenBank中BLAST比对,结果共获得29个独立的差异表达cD-NA克隆,其中有3个功能未知基因。半定量RT-PCR进一步验证了抗感病品种间抗性相关基因的差异表达。根据MIPS(Munich Information Center forProtein Sequences)功能分类系统推测,这些差异表达基因可能参与了CBB30Ⅰ对病原菌的防卫反应、信号传导和蛋白合成与修饰等一些重要的生物学过程。
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| 关 键 词： | 水稻白叶枯病 抑制消减杂交 功能分类 RT-PCR分析 |
Differential Expression Analysis of Genes Related to Rice Bacterial Blight Resistance in Rice Line CBB30Ⅰ |
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| Dong Xu,Zhang Xiaoping,Zhao Kaijun,Wang Chunlian.Differential Expression Analysis of Genes Related to Rice Bacterial Blight Resistance in Rice Line CBB30Ⅰ[J].Crops,2012(4):20-25. |
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| Authors: | Dong Xu Zhang Xiaoping Zhao Kaijun Wang Chunlian |
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| Institution: | (National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China) |
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| Abstract: | Rice bacterial blight(BB) is one of the most serious diseases of rice.Wild rice germplasm Y238 has a broad-spectrum resistance to bacterial blight.Introgression lines CBB30Ⅰ derived from cross and backcrosses between Y238,the BB resistance donors,and the susceptible cultivar IR24,the recurrent parent,also has the broad-spectrum resistance.In this study,differentially expressed cDNA library between CBB30Ⅰ and IR24 was constructed by using suppression subtractive hybridization(SSH).BB-resistance related genes were analyzed by using RT-PCR.Twenty-nine unique cDNA clones showing differential expressions were found after reverse Northern blot analysis,sequencing and BLAST analysis.Three of the twenty-nine unique cDNA clones were predicted to encode proteins of unknown function in GenBank.Differential expressions of the BB-resistance related genes in incompatible and compatible interactions were further proved by semi-quantitative RT-PCR.According to MIPS(Munich Information Center for Protein Sequences) functional classification system,the differential expression genes were involved in some important biological processes,such as pathogen response,signal transduction,protein synthesis and protein fate etc. |
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| Keywords: | Rice bacterial blight Suppression subtractive hybridization Functional classification RT-PCR analysis |
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