| 黄花菜多酚提取工艺及抗氧化作用的研究 |
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| 引用本文： | 周向军,高义霞,张继.黄花菜多酚提取工艺及抗氧化作用的研究[J].作物杂志,2012,28(1):68-72. |
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| 作者姓名： | 周向军 高义霞 张继 |
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| 作者单位： | 1. 天水师范学院生命科学与化学学院 741001 甘肃天水
2. 天水师范学院生命科学与化学学院 741001 甘肃天水;西北师范大学生命科学学院 730070 甘肃兰州 |
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| 基金项目： | 天水师范学院重点学科支持项目(ZD08037);地震灾后新农村建设技术集成与示范(2008BAR51B05) |
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| 摘 要： | 探讨黄花菜多酚最佳提取工艺及抗氧化作用。结果表明,黄花菜多酚最佳提取工艺为:提取时间60min、乙醇浓度55%、料液比1∶15、提取温度35℃;在此条件下,多酚得率为28.89%。抗氧化试验表明黄花菜多酚对羟自由基、超氧阴离子自由基、DPPH自由基均具有一定的清除作用,其半抑制率(IC50)分别为0.1176mg/mL、0.1638mg/mL和0.1593mg/mL,且清除率与黄花菜多酚浓度成量效关系。
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| 关 键 词： | 黄花菜 多酚 提取工艺 抗氧化 |
Optimization of Extraction Technology and Analyses on Antioxidant Activities of Polyphenol from Hemerocallis Citrina Baron L. |
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| Zhou Xiangjun
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Gao Yixia
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Zhang Ji.Optimization of Extraction Technology and Analyses on Antioxidant Activities of Polyphenol from Hemerocallis Citrina Baron L.[J].Crops,2012,28(1):68-72. |
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| Authors: | Zhou Xiangjun Gao Yixia Zhang Ji |
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| Institution: | 1,2(1College of Life Science and Chemistry,Tianshui Normal University,Tianshui 741001,Gansu; 2Department of Life Science,Northwest Normal University,Lanzhou 730070,Gansu,China) |
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| Abstract: | Extraction technology of polypolyphenol from Hemerocallis citrina Baron L.was studied and antioxidant activities were also analyzed.The optimized condition was as follows:extraction time 60 min,ethanol concentration at 55% with solid to liquid ratio of 1∶ 15,temperature at 35℃.Under these conditions,polyphenol content is 28.89%.Antioxidant experiments showed that polyphenol had good scavenging effect on hydroxyl radical,superoxide anion and DPPH radical with IC50 were 0.1176mg/mL,0.1638mg/mL and 0.1593mg/mL,respectively.Scavenging activity has dose-effect relationship with polypolyphenol content. |
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| Keywords: | Hemerocallis citrina Baron L Polyphenol Extraction technology Antioxidant activities |
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