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| 作为土壤污染生物标志物的蚯蚓体腔细胞提取方法比较 | |
| 引用本文： | 朱江,杨道丽,李志刚,王振旗,毛俊亚.作为土壤污染生物标志物的蚯蚓体腔细胞提取方法比较[J].上海交通大学学报(农业科学版),2011,29(3):1-5. |
| 作者姓名： | 朱江 杨道丽 李志刚 王振旗 毛俊亚 |
| 作者单位： | 1. 上海市环境科学研究院,上海,200233 2. 上海交通大学环境科学与工程学院,上海,200240 3. 山东丝绸纺织职业学院,山东淄博,255300 |
| 基金项目： | 国家自然科学基金项目(21047001); 上海市自然科学基金项目(09ZR1426700); 上海市技术标准专项(10DZ0503900) |
| 摘 要： | 比较了纤维针法（FNE）、电压刺激法（EE）、超声波刺激法（UP）、纤维针与超声波刺激结合法（CM）四种蚯蚓体腔细胞提取方法。结果表明：单位体重细胞数由大到小依次为CM〉UP〉EE〉FNE,细胞的成活率由大到小依次为CM〉FNE〉EE〉UP,其中CM法提取的单位体重细胞数（106个）较多（4.638±0.138）且存活率（%）最高（97.258±0.161）。CM法所提取的体腔细胞能满足作为土壤污染风险评估生物标志物的要求,可以为在细胞水平、亚细胞水平和分子水平对污染土壤开展风险评估提供技术支持。 |
| 关 键 词： | 蚯蚓体腔细胞 纤维针与超声波刺激结合法 生物标志物 土壤污染 |
Comparison Studies on the Methodology of Extracting Coelomcytes in Earthworm Used as a Biomarker for the Evaluation of the Soil Pollution |
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| ZHU Jiang,YANG Dao-li,LI Zhi-gang,WANG Zhen-qi,MAO Jun-ya.Comparison Studies on the Methodology of Extracting Coelomcytes in Earthworm Used as a Biomarker for the Evaluation of the Soil Pollution[J].Journal of Shanghai Jiaotong University (Agricultural Science),2011,29(3):1-5. | |
| Authors: | ZHU Jiang YANG Dao-li LI Zhi-gang WANG Zhen-qi MAO Jun-ya |
| Institution: | ZHU Jiang1,YANG Dao-li2,LI Zhi-gang3,WANG Zhen-qi1,MAO Jun-ya1(1.Shanghai Academy of Environmental Sciences,Shanghai 200233,China,2.School of Environmental Science and Engineering,Shanghai Jiaotong University,Shanghai 200240,3.Shandong Silk Textile Vocational College,Shandong Zibo 255300,China) |
| Abstract: | Four methods for extracting coelomcytes in earthworms(Eisenia foetida) were developed including FNE(fiber needle extraction),EE(electrical extraction),UP(ultrasound protocol)and CM(combined method of fiber needle extraction with ultrasound protocol).The effect of different methods on the efficiency of the extraction was compared based on the total counts of coelomcytes and viability of coelomcytes.The results showed that the ratio of coelomocyte counts to body weight from different methods was listed in the... |
| Keywords: | earthworm coelomcytes combined method of fiber needle extraction with vltrasound protocol(CM) biomarker soil pollution |
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