| 抗鸡传染性支气管炎病毒单链抗体间接ELISA筛选方法的建立 |
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| 引用本文： | 赵蕾,林源,李本强,朱建国.抗鸡传染性支气管炎病毒单链抗体间接ELISA筛选方法的建立[J].上海交通大学学报(农业科学版),2011,29(2):64-68. |
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| 作者姓名： | 赵蕾 林源 李本强 朱建国 |
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| 作者单位： | 上海交通大学农业与生物学院,上海市兽医生物重点实验室,上海200240 |
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| 基金项目： | “上海交大-美国Raybiotech联合开展抗体工程研究”科技合作项目 |
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| 摘 要： | 建立一种筛选特异性单链抗体的间接ELISA优化方法.用鸡传染性支气管炎病毒IBV-M41株接种SPF鸡胚,纯化后作为抗原包被96孔板进行间接ELISA,从本实验室构建的原核表达质粒pOPE101-XPscFv/JM109(DE3)中筛选出针对IBV-M41株的scFv,并对各反应参数进行探索,优化筛选方法.通过敏感性实...
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| 关 键 词： | 单链抗体 传染性支气管炎 间接ELISA 筛选 |
| 收稿时间： | 2011/1/24 0:00:00 |
Establishment of an Indirect ELISA Screening Method for the Detection of the Single-chain Fragments Variable Antibodies Against Infectious Bronchitis Virus |
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| ZHAO Lei,LIN Yuan,LI Ben-qiang and ZHU Jian-guo.Establishment of an Indirect ELISA Screening Method for the Detection of the Single-chain Fragments Variable Antibodies Against Infectious Bronchitis Virus[J].Journal of Shanghai Jiaotong University (Agricultural Science),2011,29(2):64-68. |
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| Authors: | ZHAO Lei LIN Yuan LI Ben-qiang and ZHU Jian-guo |
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| Institution: | ZHAO Lei,LIN Yuan,LI Ben-qiang,ZHU Jian-guo(Key Laboratory of Veterinary Biology,School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200240,China) |
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| Abstract: | In order to further develop single-chain fragments variable(scFv) against Infectious Bronchitis Virus M41 isolate(IBV-M41),we established an indirect ELISA method for screening scFv,which could specifically bind with IBV-M41.IBV-M41 isolate was inoculated into SPF chicken embryos.ELISA plates were coated with purified IBV-M41 and specific scFvs were screened.The ELISA reaction parameters were studied and optimized.The optimized conditions for this technique were as follows:IBV-M41 was diluted by 20 fold(61.... |
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| Keywords: | single-chain fragment variable(scFv) avian infectious bronchitis virus(IBV) indirect enzyme-linked immuno sorbent assay(ELISA) screen |
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