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| 沙田柚遗传转化再生体系的建立 | |
| 引用本文： | 胡新喜,敖小平,邓子牛,熊兴耀.沙田柚遗传转化再生体系的建立[J].中国农学通报,2010,26(7):177-180. |
| 作者姓名： | 胡新喜 敖小平 邓子牛 熊兴耀 |
| 作者单位： | 1. 湖南省作物种质创新与资源利用重点实验室,长沙,410128;湖南农业大学园艺园林学院,长沙,410128 2. 湖南农业大学园艺园林学院,长沙,410128 3. 湖南省作物种质创新与资源利用重点实验室,长沙,410128;国家柑橘改良中心长沙分中心,长沙,410128 |
| 基金项目： | 国家转基因植物研究与产业化开发专项 |
| 摘 要： | 以沙田柚的实生苗不同外植体为对象，进行了沙田柚遗传转化再生体系建立研究。研究结果表明：以实生苗上胚轴作为外植体，以MS无机盐＋100 mg/L肌醇＋0.2 mg/L维生素B1+1 mg/L维生素B6+1 mg/L烟酸+3％蔗糖＋0.8％琼脂（pH5.8）＋5 mg/L 6-BA为不定芽诱导培养基，暗培养7 d后，转移至光/暗周期16/8 h的条件下培养，不定芽的分化率高达91.1%；不定芽在1/2MS基本培养基＋3％蔗糖＋0.7％琼脂＋1.5 mg/L IBA＋0.2%活性炭的生根培养基，生根率高达80％；以卡那霉素作选择剂时,选择浓度为50mg/L。 |
| 关 键 词： | 辽宁地区菜豆炭疽病生理小种初鉴报告 辽宁地区菜豆炭疽病生理小种初鉴报告 |
| 收稿时间： | 2009-11-26 |
| 修稿时间： | 2009-12-13 |
Establishment of Regeneration System for Genetic Transformation of Citrus grandis Osbeck cv. Shatianyou |
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| Hu Xinxi,Ao Xiaoping,Deng Ziniu,Xiong Xingyao.Establishment of Regeneration System for Genetic Transformation of Citrus grandis Osbeck cv. Shatianyou[J].Chinese Agricultural Science Bulletin,2010,26(7):177-180. | |
| Authors: | Hu Xinxi Ao Xiaoping Deng Ziniu Xiong Xingyao |
| Institution: | 1Hunan Provincial Key Laboratory for Germplasm Innovation and Utilization of Crop, Changsha 410128; 2College of Horticulture and Landscape, HNAU, Changsha 410128; 3National Citrus Breeding Center Changsha Branch, Changsha 410128) |
| Abstract: | Transgenic plants appear to be a new way to citrus improvement and a powerful tool for studying gene functions. The establishment of an efficient in vitro plant regeneration system suitable for genetic transformation is the first step in this approach. Different explants of seedlings were grownin vitro and a efficient regeneration system was established for Citrus grandis Osbeck cv. Shatianyou. The results were obtained as follow. When the epicotyl pieces were explants were cultured on the adventitious shoot regeneration medium （MS salts ＋100 mg/L inositol ＋0.2 mg/LV-B1＋1 mg/LV-B6＋1 mg/L niacin ＋ 3% sucrose ＋ 5 mg/L6- BA ＋ 0.8% agar,pH5.8） and incubated in darkness for 7 days,then in the condition of 16/8 h light/ darkness cycle,adventitious shoot regenerated from 91.1% of the epicotyl explants. When the adventitious shoots were cultured on the medium （1/2MS＋1.5 mg/L IBA ＋3%sucrose ＋0.7%agar,pH5.8） , adventitious root regenerated from 80% of the adventitious shoot after grown in vitro for 40d. As a selectable agent, concentration of Kanamycin is 50 mg/L for epicotyl pieces. |
| Keywords: | Citrus grandis Osbeck cv Shatianyou transformation in vitro regeneration |
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