| 阳离子交换树脂固定化乳糖酶的研究 |
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| 引用本文： | 王静,贾英民,王华,鲁凤娟.阳离子交换树脂固定化乳糖酶的研究[J].安徽农业科学,2010,38(1):327-328,331. |
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| 作者姓名： | 王静 贾英民 王华 鲁凤娟 |
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| 作者单位： | 1. 中国环境管理干部学院生态学系,河北秦皇岛,066004
2. 河北农业大学食品科技学院,河北保定,071001 |
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| 基金项目： | 河北省农副产品加工重大攻关专项项目(03220173D) |
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| 摘 要： | 目的]以阳离子交换树脂为载体，研究黑曲霉来源乳糖酶固定化条件，以期改善乳糖酶性质，使黑曲霉乳糖酶更适于低乳糖乳的连续生产。方法]以吸附率最高的阳离子交换树脂D151为载体，通过先吸附后交联的方法固定乳糖酶，优化固定化条件。结果]结果表明，加酶量为50．0U／g（载体），吸附pH值为4．0，吸附温度是25℃，吸附时间24h，交联剂戊二醛浓度为4％，交联温度是30℃，交联时间是6h，固定化效果最好。获得的固定化酶活力可达11．8U／g（载体），固定酶回收率为37．2％。结论]该研究为工业化利用固定化乳糖酶连续生产低乳糖乳提供了技术依据。
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| 关 键 词： | 乳糖酶 阳离子交换树脂 固定化 载体 |
Research on the Immobilization of Lactase with Cation Exchange Resin |
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| WANG Jing et al.Research on the Immobilization of Lactase with Cation Exchange Resin[J].Journal of Anhui Agricultural Sciences,2010,38(1):327-328,331. |
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| Authors: | WANG Jing |
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| Institution: | Department of Ecology;China Environmental Management College;Qinhuangdao;Hebei 066004 |
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| Abstract: | Objective] The cation exchange resin being taken as the carrier,the immobilization condition of the lactase derived from aspergillus niger was researched so that the lactase from aspergillus niger was more suitable for the continuous production of low-lactose milk through the improvement of the property of lactase.Method] The cation exchange resin-D151 with the highest absorption rate being taken as the carrier,the immobilization condition was optimized through the experiment in the method of cross-linkin... |
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| Keywords: | Lactase Cation exchange resin Immobilization Carrier |
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