|
|||||
|
|
| 柱状黄杆菌外膜蛋白的比较蛋白质组学研究 | |
| 引用本文： | 刘国勇,陈发菊,聂品.柱状黄杆菌外膜蛋白的比较蛋白质组学研究[J].华中农业大学学报,2007,26(6):827-830. |
| 作者姓名： | 刘国勇 陈发菊 聂品 |
| 作者单位： | 1. 三峡大学化学与生命科学学院,宜昌,443002;三峡大学生物技术研究中心,宜昌,443002 2. 三峡大学生物技术研究中心,宜昌,443002 3. 淡水生态与生物技术国家重点实验室/中国科学院水生生物研究所,武汉,430072 |
| 基金项目： | 国家自然科学基金项目(30025035)，三峡大学博士启动基金资助 |
| 摘 要： | 为了鉴定柱状黄杆菌(Flavobacterium columnare)的毒力相关因子,利用蛋白质组学方法分析了强毒株G4R3和弱毒株G18的外膜蛋白。双向电泳结合图像分析,共发现了8个差异表达的蛋白点。经过胶内酶解和质谱分析,其中的1个蛋白点被鉴定为LemA蛋白,它可能是柱状黄杆菌的一种毒力因子。 |
| 关 键 词： | 柱状黄杆菌 毒力因子 外膜蛋白 双向电泳 质谱 |
| 文章编号： | 1000-2421（2007）06-0827-04 |
| 收稿时间： | 2007-04-26 |
| 修稿时间： | 2007年4月26日 |
Comparative Proteomic Analysis of Outer Membrane Proteins of Flavobacterium columnare Strains |
|
| LIU Guo-yong,CHEN Fa-ju,NIE Pin.Comparative Proteomic Analysis of Outer Membrane Proteins of Flavobacterium columnare Strains[J].Journal of Huazhong Agricultural University,2007,26(6):827-830. | |
| Authors: | LIU Guo-yong CHEN Fa-ju NIE Pin |
| Abstract: | In this study,a proteome approach was carried out to compare the outer membrane proteins of virulent strains and avirulent strains of Flavobacterium columnare in order to identify the virulence factors of the pathogen.Proteomic analysis by two-dimensional polyacrylamide gel electrophoresis(2-DE) was applied to analyze the outer membrane proteins of G4R3 and G18 at the late exponential growth phase.8 differential expression protein spots between the two strains were revealed by image analysis of 2-DE gels,and they were further analyzed by using in-gel tryptic digestion and mass spectrometry.One spot was identified as LemA,which was considered as a virulence-related factor of F.columnare. |
| Keywords: | Flavobacterium columnare virulence factors outer membrane proteins two-dimensional polyacrylamide gel electrophoresis mass spectrometry |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |
| 点击此处可从《华中农业大学学报》浏览原始摘要信息 | |
| 点击此处可从《华中农业大学学报》下载免费的PDF全文 | |