A new disease of greenhouse tomatoes in Israel caused by a distinct strain ofTomato apical stunt viroid (TASVd) |
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| Authors: | Y Antignus O Lachman M Pearlsman R Gofman M Bar-Joseph |
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| Institution: | (1) Dept. of Virology, ARO, The Volcani Center, 50250 Bet Dagan, Israel |
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| Abstract: | Using the sequential PAGE method for detection of small circular RNA molecules we isolated a viroid from greenhouse-grown
tomato plants exhibiting severe stunting in Israel. The viroid was transmitted to tomato and to several other solanaceous
plants by graft and mechanical inoculation, but only tomato plants showed symptoms of disease. Cloning and sequencing revealed
that the viroid RNA is composed of 363 nucleotides, has 92% identity with the type strain (Ivory Coast strain) ofTomato apical stunt viroid (TASVd) and 99% identity with the Indonesian strain of this viroid. The experimental host range of TASVd-Is differs significantly
from that of the type strain of TASVd. The possible epidemiological consequences leading to TASVd spread in geographically
distant areas are discussed.
http://www.phytoparasitica.org posting Sept. 18, 2002.
Corresponding author |
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| Keywords: | Host range purification full sequence molecular probe diagnostics |
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