| 鸡白细胞介素2口服免疫佐剂增强传染性法氏囊病病毒DNA疫苗免疫效果的研究 |
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| 引用本文： | 谢荣辉,万旺军,李龙,李建荣,于涟.鸡白细胞介素2口服免疫佐剂增强传染性法氏囊病病毒DNA疫苗免疫效果的研究[J].浙江大学学报(农业与生命科学版),2007,33(3):237-242. |
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| 作者姓名： | 谢荣辉 万旺军 李龙 李建荣 于涟 |
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| 作者单位： | 1. 浙江大学,动物预防医学研究所,浙江,杭州,310029;浙江省疾病预防控制中心病毒所,浙江,杭州,310009
2. 浙江大学,动物预防医学研究所,浙江,杭州,310029 |
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| 基金项目： | 浙江省科技计划;浙江省自然科学基金 |
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| 摘 要： | 将含萧山鸡白细胞介素2基因的真核表达质粒pCI-IL-2的减毒鼠伤寒沙门氏茵ZJ111(ZJ111/pCI-IL-2)口服接种小鼠和雏鸡,并与传染性法氏囊病病毒(IBDV)DNA疫苗联合免疫雏鸡观察其免疫效力.结果表明:利用减毒沙门氏菌作为栽体的鸡白细胞介素2口服免疫增强剂具有相对的安全性,重组ZJ111/pCI-IL-2茵能明显增强IBDV DNA疫苗对强毒株攻击保护率;增强IBDV DNA疫苗诱导的抗体的效价(P＜0.05);增强IBDV DNA疫苗所诱导的T淋巴细胞和B淋巴细胞的增殖反应(P＜0.05),上述结果初步表明以减毒沙门氏菌为载体的白细胞介素2免疫增强剂具有良好的安全性和免疫增强作用,为研制低成本、实用化的禽类口服免疫增强剂奠定了基础.
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| 关 键 词： | 鸡白细胞介素2 传染性法氏囊病病毒 免疫增强 |
| 文章编号： | 1008-9209（2007）03-0237-06 |
| 修稿时间： | 2005-01-15 |
Enhancement of immunogenicity of DNA vaccine against infectious bursal disease virus by co-delivery with oral vaccine encoding chicken interleukin-2 |
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| XIE Rong-hui,WAN Wang-jun,LI Long,LI Jian-rong,YU Lian.Enhancement of immunogenicity of DNA vaccine against infectious bursal disease virus by co-delivery with oral vaccine encoding chicken interleukin-2[J].Journal of Zhejiang University(Agriculture & Life Sciences),2007,33(3):237-242. |
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| Authors: | XIE Rong-hui WAN Wang-jun LI Long LI Jian-rong YU Lian |
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| Institution: | 1. Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou 310029, China; 2. Institute of Virology, Center for Disease Control and Prevention of Zhejiang Province, Hangzhou 310009, China |
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| Abstract: | Mice and 14-day-old chickens were orally inoculated with the recombinant attenuated Salmonella typhimurium strain ZJ111 carrying pCI-IL-2 expression plasmid encoding the Interleukin II protein.The results showed the ZJ111/pCI-IL-2 was relatively safe.Fourteen-day-old non-immunized chickens were co-administrated orally and intradermally with DNA vaccine and ZJ111/pCI-IL-2 at three dosage levels(107 cfu,108 cfu,109 cfu),and boosted two weeks later.Control groups(challenge control,normal control,DNA vaccine control) were included.The chickens were challenged with virulent infectious bursal disease virus(IBDV) seventeen days post-boosting.The results showed that protective responses and neutralization antibody response of DNA vaccine co-administrated with chicken interleukin-2(IL-2) were much higher than those of injected with DNA vaccine alone.Futhermore,the T lymphocyte proliferation response of thymus and spleen,and the B lymphocyte proliferation response of bursa induced by DNA vaccine can be significantly enhanced by chicken IL-2.These results obviously indicated that chicken IL-2 was a strong adjuvant can be significantly enhance the immunogenicity of IBDV DNA vaccine. |
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| Keywords: | chicken interleukin-2(IL-2) infectious bursal disease virus(IBDV) immunity enhancing |
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