| 泰山赤鳞鱼苹果酸酶同工酶表型差异的研究 |
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| 引用本文： | 庞秋香,庞书香,赵博生,陈艳艳,张萌.泰山赤鳞鱼苹果酸酶同工酶表型差异的研究[J].农业科学与技术,2009,10(5):85-87,99. |
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| 作者姓名： | 庞秋香 庞书香 赵博生 陈艳艳 张萌 |
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| 作者单位： | [1]山东理工大学生命科学院,山东淄博255049 [2]济宁职业技术学院生化系,山东济宁272037 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 应用聚丙烯酰胺凝胶电泳结合生化染色方法分别对雌雄泰山赤鳞鱼10种组织中苹果酸酶同工酶酶谱进行分析。结果表明，在所取的10种组织中，雌性泰山赤鳞鱼在心脏、性腺、肾、眼、鳃和脾6种组织中存在苹果酸酶，而雄性泰山赤鳞鱼只在心、肌肉、性腺、眼和鳃5种组织中存在苹果酸酶。雌性鱼心脏组织中只有1条ME-2带，而雄性鱼心脏组织含有ME-1和ME-22条酶带；卵巢中含有ME-1和ME-22条酶带，而精巢中只含有ME-11条带；雌性鱼的眼组织含有ME-1带，而雄性鱼眼中含有ME-2带。肾和脾组织只在雌性鱼中含有苹果酸酶酶带，而肌肉组织只在雄性鱼中含有苹果酸酶。苹果酸酶的表型在泰山赤鳞鱼不同性别同一组织和同一性别不同组织之间都存在明显差异，但在同一性别的不同个体之间不存在差异。该研究结果为赤鳞鱼种群的发育遗传学、品种改良和定向育种提供了基础资料，为赤鳞鱼的开发和保护奠定基础。
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| 关 键 词： | 泰山赤鳞鱼 苹果酸酶 同工酶 表型差异 |
Isozyme Phenotypic Difference of Varicorhinus macrolepis Malic Enzyme |
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| PANG Qiu-xiang,PANG Shu-xiang,ZHAO Bo-sheng,CHEN Yan-yan,ZHANG Meng.Isozyme Phenotypic Difference of Varicorhinus macrolepis Malic Enzyme[J].Agricultural Science ＆ Technology,2009,10(5):85-87,99. |
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| Authors: | PANG Qiu-xiang PANG Shu-xiang ZHAO Bo-sheng CHEN Yan-yan ZHANG Meng |
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| Institution: | 1, School of Life Sciences, Shandong University of Technology, Zibo 255049; 2, Biology＆Chemistry Department of Jining Vocational Technology College, Jining 272037) |
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| Abstract: | By using polyacrylamide gel electrophoresis and biochemical straining method, malic enzyme isozyme in 10 organs of male or female Varicorhinus macrolepis were analyzed. The results showed that malic enzyme exists in heart, gonads, eyes and gills tissues in both female and male V. macrolepis. There was only one ME-2 in heart and ME-1 in eye of female fish, while there were ME-1 and ME-2 in heart and ME-2 in eyes of male fish. Ovary contained ME-1 and ME-2, while spermary only contained ME-1. In kidney and spleen tissues, malic enzyme was found only in female fish, while in muscle, malic enzyme existed only in male fish. The phenotypic of malic enzyme had obvious differences no matter in the same organ of different sexes in V. macrolepis or different organs in the same sex. But there were no differences among different individuals of the same sex. The results will provide basic data for developmental genetics, breed improvement and directed breeding of V. macrolepis, and lay a foundation for its development and protection. |
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| Keywords: | Varicorhinus macrolepis Malic enzyme Isozyme Phenotypic difference |
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