| 1株广东鸡源新城疫病毒的分离鉴定和毒力基因分析 |
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| 引用本文： | 黎先伟,;钟植文,;陈珊,;吴晓禅,;刘镇明,;杨傲冰.1株广东鸡源新城疫病毒的分离鉴定和毒力基因分析[J].畜牧兽医科技信息,2014(4):34-37. |
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| 作者姓名： | 黎先伟 ;钟植文 ;陈珊 ;吴晓禅 ;刘镇明 ;杨傲冰 |
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| 作者单位： | [1]广东永顺生物制药股份有限公司,广东广州511356; [2]华南农业大学兽医学院,广东广州510642 |
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| 摘 要： | 从广东粤东地区某商品蛋鸡场的发病鸡群中分离到1株病毒（YS株）,经血凝和血凝抑制试验确定为新城疫病毒（NDV）.对该分离株的F蛋白氨基酸序列的分析结果表明,其F0裂解位点的氨基酸序列为1nR-R-Q-K-R-F117,且含有101K和121V,符合典型NDV强毒株的分子特点.遗传分析结果显示分离株属于基因Ⅶd亚型.F和HN基因的氨基酸相似性分析表明,分离株与基因Ⅶ型NDV Chicken/China/Shandong/02/2010株的相似性最高,均达到99％,而与常用疫苗株B1、V4、Clone30、Mukteswar和La Sota的相似性则较低,分别在86.8％～ 90.4％和87.2％～88.3％之间,说明分离株与经典的NDV毒株存在一定的差异.
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| 关 键 词： | 分离 基因Ⅶ型 鸡 |
Identification of One Newcastle Disease Viruse Strain and its Virulent Genes |
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| Institution: | Li Xianwei, Zhong Zhiwen, Chen Shan, Wu Xiaochan, Liu Zhenming, Yang Aobing ( 1.Guangdong Win-sun Bio-pharmaceutical Co., Ltd, Guangzhou 511356, China; 2.College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China ) |
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| Abstract: | In recent years, atypical Newcastle disease was popular in some domestic areas. Samples from some sick commercial layer from eastern Guangdong, were inoculated to 9-11 day-old chicken embryos, and one virus, named YS, was isolated. HA and HI tests showed Newcastle disease virus positive. The result of sequence analysis showed that the amino-acid sequence of the fusion protein cleavage site was 112R-R-Q-K-R-F117, with 101 K and 121 V, a typical sequence ofvelogenic strains. Genetic analysis showed that the isolate belonged to genotype VII. The homology analysis of F and HN gene showed that the isolate strain had highest identities with the genotype VII NDV （Chicken/China/Shandong/02/2010）, both with 99%, but low identities with the common vaccine strains, such as B 1, V4, Clone30, Mukteswar and LaSota, only with 86.8%- 90.4% and 87.2%-88.3%, respectively, which differed from the classical strains of NDV. |
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| Keywords: | Newcastle disease virus F gene HN gene genotype VII velogenic strain |
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