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三倍体毛白杨组培快繁技术研究
引用本文:元秀慧.三倍体毛白杨组培快繁技术研究[J].山西林业科技,2014(1):34-35.
作者姓名:元秀慧
作者单位:山西省桑干河杨树丰产林实验局,山西大同037006
摘    要:笔者从试验材料、外植体的灭菌与接种、培养条件,分化与生根培养,小植株的移栽与定植3个方面介绍了三倍体毛白杨的组培快繁技术,指出采用增殖与生根同步进行的方法进行快繁,年增殖次数可达8次,每次增殖倍数为4.4倍,培养出的组培苗质量好,移栽成活率高,基本达到了工厂化生产的指标要求。在此基础上,进行了三倍毛白杨组培快繁的成本核算,从成本核算过程中可以看出,人工费用和能源消耗费是制约苗木成本的两大因素,其中,人工费用约占全部支出的40%.

关 键 词:三倍体毛白杨  组织培养  繁殖  成本

Tissue Culture and Rapid Propagation Technology of Triploid Populus tomentosa
Yuan Xiuhui.Tissue Culture and Rapid Propagation Technology of Triploid Populus tomentosa[J].Shanxi Forestry Science and Technology,2014(1):34-35.
Authors:Yuan Xiuhui
Institution:Yuan Xiuhui ( Saagganhe Bureau of Poplar High-yield Forest, Shanxi Datoug 037006, China)
Abstract:Tissue culture and rapid propagation techniques of triploid Populus tornentosa were introduced from sterilization, inoculation and culture condition of material and explant, differentiation and rooting culture, transplanting and planting of plantlet. Using the method of simultaneous proliferation and rooting for rapid propagation, the proliferation number reached 8 times every year,the average proliferation times were 4.4, tissue culture seedling had good quality and the transplanting survival rate was high which basically reached the index requirements of factory production. On the basis, cost accounting of tissue culture and rapid propagation of triploid Populus tomentosa was evaluated. As could be seen from the process of cost accounting, labor cost and energy consumption were the two factors to restrict seedling cost, and among them, the la- bor cost accounted for about 40% of total cost.
Keywords:Triploid Populus tomentosa  Tissue culture  Propagation  Cost
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