Effect of Eperythrozoon ovis infection on the reductive potential of sheep erythrocytes |
| |
| Authors: | RH Sutton |
| |
| Institution: | Department of Veterinary Pathology and Public Health, Massey University, Palmerston North, New Zealand |
| |
| Abstract: | Reduced glutathione (GSH) and methaemoglobin were measured in sheep blood during an infection cycle of Eperythrozoon ovis and in uninfected control blood. The GSH levels in infected erythrocytes were significantly lower in the latter half of the infection cycle. Incubation with acetylphenylhydrazine (APH) resulted in negligible leves in infected cells whereas control cells were approximately 45% normal. Infection with E. ovis had little effect on methaemoglobin levels. However, incubation with APH caused a marked increased of the methaemoglobin levels in both infected and control blood; the control methaemoglobin levels were significantly higher.It was concluded from these observations that E. ovis interfered with maintenance of GSH within erythrocytes to an extent that when challenged with an oxidizing chemical erythrocyte membrane integrity could be lost. In addition E. ovis may bave provided some protection against oxidative damage for the heme portion of the haemoglobin molecule or the amount of oxidative damage to haemoglobin was further advanced in the infected cells. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
