| 簇毛麦易位系V9125-2抗条锈基因YrWV的遗传分析和SSR分子标记 |
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| 引用本文： | 王睿,张书英,徐中青,陈洁,李强,侯璐,井金学.簇毛麦易位系V9125-2抗条锈基因YrWV的遗传分析和SSR分子标记[J].中国农业科学,2011,44(1):9-19. |
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| 作者姓名： | 王睿 张书英 徐中青 陈洁 李强 侯璐 井金学 |
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| 作者单位： | (西北农林科技大学植物保护学院/陕西省农业分子生物学重点实验室); |
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| 基金项目： | 教育部高等学校学科创新引智计划(B07049); 科技部小麦重大病虫害防控技术(2006BAD08A05); 农业部小麦条锈病菌毒性变异与条锈病综合治理研究示范项目(200903035-02) |
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| 摘 要： | 目的]对高抗条锈病的簇毛麦易位系V9125-2进行研究,明确其抗病性遗传特点,并对其抗条锈病基因定位,为选育优质抗源材料提供依据.方法]采用中国当前流行的7个条锈菌生理小种CYR29、CYR30、CYR31、CYR32、CYR33以及Su11-4、Su11-11对簇毛麦易位系V9125-2和铭贤169的杂交后代进行...
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| 关 键 词： | 簇毛麦 普通小麦 易位系 条锈病 遗传分析 SSR标记 |
| 收稿时间： | 2010-05-20; |
Genetic Analysis and SSR Molecular Mapping of New Stripe-Rust Resistance Gene YrWV Derived from Triticum aestivum-Haynaldia villosa Translocation Line V9125-2 |
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| WANG Rui,ZHANG Shu-ying,XU Zhong-qing,CHEN Jie,LI Qiang,HOU Lu,JING Jin-xue.Genetic Analysis and SSR Molecular Mapping of New Stripe-Rust Resistance Gene YrWV Derived from Triticum aestivum-Haynaldia villosa Translocation Line V9125-2[J].Scientia Agricultura Sinica,2011,44(1):9-19. |
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| Authors: | WANG Rui ZHANG Shu-ying XU Zhong-qing CHEN Jie LI Qiang HOU Lu JING Jin-xue |
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| Institution: | WANG Rui1,ZHANG Shu-ying1,XU Zhong-qing1,CHEN Jie2,LI Qiang1,HOU Lu1,JING Jin-xue1(1College of Plant Protection,Northwest Agriculture and Forestry University/Shaanxi Key Laboratory of Molecular Biology for Agriculture,Yangling 712100,Shaanxi,2Institute of Plant Protection,Hebei Academy of Agricultural and Forestry Sciences,Baoding 071000,Hebei) |
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| Abstract: | 【Objective】 The high stripe rust resistance material, translocation line V9125-2, from Triticum aestivum-Haynaldia villosa was studied. Inheritance, location and SSR molecular mapping of stripe rust resistance genes in V9125-2 are important for providing a basis for breeding high stripe rust resistance materials. 【Method】 To identify and tag new resistance genes to stripe rust in the translocation line V9125-2 from Triticum aestivum-Haynaldia villosa, the yellow rust races(Puccinia striiform f.sp. stritici)CYR29, CYR30, CYR31, CYR32, CYR33, Su11-4 and Su11-11 were selected as innoculum of genetic analysis of resistance to stripe rust in the progeny population of V9125-2×Mingxian169 at seedling stage. Using the F2 population inoculated with CYR29 to test SSR analysis, combined with bulked segregation analysis (BSA), 289 pairs of primers were used. The F3 population were used to test the linked markers. And the homologous rate between the stripe rust resistance genes of V9125-2 and the varieties in Huang-Huai wheat grown region was detected. 【Result】 The results showed that one dominant gene conferred resistance to stripe rust race CYR29. One dominant gene and one recessive gene indenpently conferred to CYR30, CYR32, CYR33 and Su11-11. And two pairs of independent dominant genes conferred to CYR31 and Su11-4, respectively. The F2 population inoculated with CYR29 were used for SSR analysis, temporarily designated as YrWV. SSR markers, as well as 289 pair primers, combined with bulked segregation analysis (BSA) revealed that six SSR markers, Xbarc87, Xwmc463, Xwmc405, Xbarc126, Xwmc438 and Xgwm473, located on chromosome 7DS were linked to YrWV, and their genetic distances to YrWV were 9.1, 3.9, 5.1, 12.6, 29.0 and 57.4cM, respectively, and the six sites linked to YrWV were validated by a segregated ratio of F3 lines. These markers would be used for breeding new wheat cultivars with marker-assisted selection. A very low homologous rate was found in 43 varieties in Huang-Huai wheat grown region tested with YrWV tags. 【Conclusion】 The result realed that the six SSR markers, Xbarc87, Xwmc463, Xwmc405, Xbarc126, Xwmc438 and Xgwm473, located on the short arm of chromosome 7D in wheat were linked to YrWV. It may be a new wheat stripe rust resistant gene which comes from Haynaldia villosa. |
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| Keywords: | Haynaldia villosa common wheat(Triticum aestivum) translocation lines stripe rust genetic analysis SSR marker |
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