| 盐胁迫对桑树NH_4~+同化和谷氨酰胺合成酶活性的影响 |
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| 引用本文： | 苏国兴,宋卫平,洪法水.盐胁迫对桑树NH_4~+同化和谷氨酰胺合成酶活性的影响[J].蚕业科学,2003,29(1):90-94. |
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| 作者姓名： | 苏国兴 宋卫平 洪法水 |
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| 作者单位： | 苏州大学生命科学学院生物科学系,苏州,215006 |
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| 摘 要： | 以育 15 1和新一之 2个桑品种的硬枝扦插苗为材料 ,研究外源NaCl胁迫对桑树氮素代谢有关酶活性和氨积累的影响。结果表明 :NaCl胁迫显著抑制桑树的营养生长 ;可促进游离NH+4在桑树叶片中积累 ,但NH+4在根系中未见明显变化 ;受NaCl胁迫的桑树根和叶片的谷氨酰胺合成酶的活性明显下降 ,而叶片的谷氨酸脱氢酶的活性显著增加 ;桑树根系硝酸还原酶的活性受盐胁迫的刺激 ,但桑树叶片的硝酸还原酶活性明显下降 ;暗示盐分胁迫可减弱桑树经谷氨酰胺合成酶 -谷氨酸合酶循环对NH+4的同化 ,但可加强NH+4经谷氨酸脱氢酶的同化 ,并可通过影响NO-3 的还原来影响桑树的氮素营养
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| 关 键 词： | 桑树 NH+4同化 谷氨酰胺合成酶 谷氨酸脱氢酶 NaCl胁迫 |
| 文章编号： | 0257-4799(2003)01-0090-05 |
| 修稿时间： | 2002年5月27日 |
Influence of NaCl Stress on NH+4 Assimilation and Activity of Glutamine Synthetase of Two Mulberry Species |
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| SU Guoxing,SONG Weiping,HONG Fashui.Influence of NaCl Stress on NH+4 Assimilation and Activity of Glutamine Synthetase of Two Mulberry Species[J].Acta Sericologica Sinica,2003,29(1):90-94. |
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| Authors: | SU Guoxing SONG Weiping HONG Fashui |
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| Abstract: | The effects of exogenous NaCl stress on the enzymes related to nitrogen metabolism and ammonium accumulations were studied in two mulberry species seedlings (Shinichinose and Yu151)cultivated in sands by shoot cuttage.The results showed that NaCl stress obviously inhibited the vegetative growth of two mulberry species,led to NH + 4 accumulation in mulberry leaves,while little changes in roots,and resulted in decreases in activities of glutamine synthetase both in leaves and roots,obvious increases in activities of glutamate dehydrogenase in leaves.The activity of nitrate reductase extracted from roots was stimulated at any rates under NaCl stress,while that in leaves was apparently declined.The consequences suggested that NaCl stress could affect the nitrogen metabolism of mulberry in salinity through weakening NH + 4 assimilation via the glutamine synthetase glutamate synthase cycle,enhancing the pathway of glutamate dehydrogenase,and influencing NO - 3 reduction. |
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| Keywords: | Mulberry NH + 4 assimilation Glutamine synthetase Glutamate dehydrogenase NaCl stress |
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