| 鹅掌楸Genomic-SSR反应体系优化 |
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| 引用本文： | 贾波,徐海滨,徐阳,龙晓飞,陈金慧,施季森.鹅掌楸Genomic-SSR反应体系优化[J].林业科学研究,2013,26(4):506-510. |
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| 作者姓名： | 贾波 徐海滨 徐阳 龙晓飞 陈金慧 施季森 |
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| 作者单位： | 1. 南京林业大学林木遗传与生物技术省部共建教育部重点实验室,江苏南京,210037
2. 中国医学科学院北京协和医学院药用植物研究所濒危药材繁育国家工程实验室,北京,100193 |
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| 基金项目： | 国家高技术研究发展计划(863计划)资助(课题编号:2013AA102705),国家自然基金(31170619) |
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| 摘 要： | The optimization of the Genomic-SSR reaction system is a basic protocol when the Genomic-SSR is used for genetic analysis in Liriodendron. The concentrations of Mg2+, dNTP, Primer and rTaq were tested by L9(34) orthogonal experiment and single factor gradient experiment to gain the optimal reaction system. The results indicated that the optimal reaction system should contain 75 ng of genomic DNA, 1 μL of 10×buffer, 0.4 μL of 10 mmol·L-1 dNTP, 0.75 μL of 2.5 mmol·L-1 MgCl2, 0.25μL of 10 μmol·L-1 Primer, 0.05 μL of 5 U rTaq and final volume of 10μL. Repeated trials and two verification tests showed that this optimal reaction system was stable, reliable, efficient and suitable for the applications of Genomic-SSR in Liriodendron population genetics and quantitative genetics research.
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| 关 键 词： | Genomic-SSR 鹅掌楸 正交试验 单因素梯度试验 |
| 收稿时间： | 2/8/2013 12:00:00 AM |
Optimization of Genomic-SSR Reaction System in Liriodendron |
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| JIA Bo,XU Hai-bin,XU Yang,LONG Xiao-fei,CHEN Jin-hui and SHI Ji-sen.Optimization of Genomic-SSR Reaction System in Liriodendron[J].Forest Research,2013,26(4):506-510. |
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| Authors: | JIA Bo XU Hai-bin XU Yang LONG Xiao-fei CHEN Jin-hui and SHI Ji-sen |
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| Institution: | Key Laboratory of Forest Genetics & Biotechnology of Ministry of Education, Nanjing Forestry University, Nanjing 210037, Jiangsu, China;The National Engineering Laboratory for Breeding of Endangered Medicinal Materials, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China;Key Laboratory of Forest Genetics & Biotechnology of Ministry of Education, Nanjing Forestry University, Nanjing 210037, Jiangsu, China;Key Laboratory of Forest Genetics & Biotechnology of Ministry of Education, Nanjing Forestry University, Nanjing 210037, Jiangsu, China;Key Laboratory of Forest Genetics & Biotechnology of Ministry of Education, Nanjing Forestry University, Nanjing 210037, Jiangsu, China;Key Laboratory of Forest Genetics & Biotechnology of Ministry of Education, Nanjing Forestry University, Nanjing 210037, Jiangsu, China |
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| Abstract: | |
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| Keywords: | Genomic-SSR Liriodendron orthogonal experiment single factor gradient experiment |
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