Anti-oxidant status in embryonic,post-hatch and larval stages of Asian seabass (<Emphasis Type="Italic">Lates calcarifer</Emphasis>) |
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| Authors: | N Kalaimani N Chakravarthy R Shanmugham A R Thirunavukkarasu S V Alavandi T C Santiago |
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| Institution: | (1) Central Institute of Brackishwater Aquaculture, 75, Santhome High Road, R.A. Puram, Chennai, Tamil Nadu, 600028, India |
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| Abstract: | The concentrations of anti-oxidant enzymes such as superoxide dismutase (SOD), catalase (CAT) and selenium-dependent glutathione
peroxidase (SeGPx), and low molecular weight free-radical scavengers such as reduced glutathione (GSH) and ascorbic acid (vitamin C)
were evaluated during the period from gastrulation (GS) to 25 days post-hatch (dph) in the larvae of Asian Seabass, Lates calcarifer. Oxidative damage due to lipid peroxidation (LPO) was also assessed, by evaluation of the formation of malondialdehyde (MDA).
All the three anti-oxidant enzymes, SOD, CAT and GPx, showed high activities during gastrulation, suggesting an increased
metabolic rate during the period of embryonic development. Though the SOD activity apparently decreased progressively during
3–20 dph of larval development, the difference was not significant. CAT showed high activity during gastrulation and remained
constant up to 3 dph, suggesting an increased need to metabolise hydrogen peroxide (H2O2) and organic peroxides. In contrast, SeGPx activity increased progressively from 5 dph to 25 dph during larval development,
indicating an increased need to detoxify lipid peroxides. This is evident from the observation of increased lipid peroxidation
from 10 dph to 25 dph during larval development. GSH levels were low at gastrulation, indicating increased metabolic rate
and formation of lipid radicals during this period, corresponding to the decrease in the level of ascorbic acid, which is
consumed for regeneration of GSH. |
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| Keywords: | Anti-oxidant enzymes Ascorbic acid Eggs Embryos Gastrulation Larvae Lates calcarifer Malondialdehyde Reactive oxygen species |
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