Genomic analysis of antifungal metabolite production by <Emphasis Type="Italic">Pseudomonas fluorescens</Emphasis> Pf-5 |
| |
| Authors: | Joyce E Loper Harald Gross |
| |
| Institution: | (1) Horticultural Crops Research Laboratory, Agricultural Research Service, US Department of Agriculture, 3420 N.W. Orchard Ave., Corvallis, OR, USA;(2) Institute for Pharmaceutical Biology, University of Bonn, Nussallee 6, 53115 Bonn, Germany |
| |
| Abstract: | The complete genomic sequences of several Pseudomonas spp. that inhabit the rhizosphere are now available, providing a new opportunity to advance knowledge of plant growth-promoting
rhizobacteria (PGPR) through genomics. Among these is the biological control bacterium Pseudomonas fluorescens Pf-5. Nearly 6% of the 7.07 Mb genome of Pf-5 is devoted to the biosynthesis of secondary metabolites, including antibiotics
toxic to soilborne fungi and Oomycetes that infect plant roots, and two siderophores involved in iron acquisition. Three orphan
gene clusters, for which the encoded natural product was unknown, also were identified in the genome of Pf-5. The product
synthesized from one of the orphan gene clusters was identified recently using a new ‘genomisotopic approach’, which employs
a combination of genomic sequence analysis and isotope guided fractionation. Application of the genomisotopic approach to
one orphan gene cluster in Pf-5 resulted in the discovery of orfamide A, founder of a new group of bioactive cyclic lipopeptides
with a putative role in biological control of plant disease. |
| |
| Keywords: | Cyanide Cyclic lipopeptides 2 4-Diacetylphloroglucinol Orphan gene clusters Pyrrolnitrin Pyoluteorin Mcf toxin |
| 本文献已被 SpringerLink 等数据库收录！ |
|
