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| 甜菜RAPD反应体系优化及亲缘关系研究 | |
| 引用本文： | 腊萍,罗淑萍,章建新,郭长奎,刘焕霞,麻浩,赵图强.甜菜RAPD反应体系优化及亲缘关系研究[J].新疆农业大学学报,2010,33(1):1-6. |
| 作者姓名： | 腊萍 罗淑萍 章建新 郭长奎 刘焕霞 麻浩 赵图强 |
| 作者单位： | 1. 新疆农业大学,农学院,乌鲁木齐,830052 2. 石河子甜菜研究所,石河子,832011 3. 南京农业大学,南京,210095 |
| 基金项目： | 新疆自治区高技术项目，新疆自治区教育厅重点项目 |
| 摘 要： | 利用正交试验设计,从Mg~(2+)、dNTP、引物、Taq酶和模板5种因素5个水平上对甜菜RAPD反应体系进行优化,确立了适合甜菜RAPD反应体系:25μL反应体系中含1×Buffer、1.0 mmol/LMg~(2+)、1U Taq DNA聚合酶、0.25 mmol/LdNTP、0.4μmol/L随机引物和25 ngDNA模板。利用优化的RAPD反应体系对38个甜菜品种进行亲缘关系分析。结果表明,38个品种之间的多态性比较高,变异大。聚类图结果表明,38个甜菜品种遗传距离的变异范围在0.14～0.72。当以遗传距离0.50来划分时,可将38个品种分为两大类,以遗传距离0.40来划分时,又可以把第2大类分为6个亚类。结果显示,RAPD优化体系适合甜菜亲缘关系鉴定。 |
| 关 键 词： | 甜菜 RAPD 正交设计 亲缘关系 |
Study on Optimization for RAPD Reaction System and Genetic Relationship in Beta vulgaris |
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| LA Ping,LUO Shu-ping,ZHANG Jian-xin,GUO Chang-kui,LIU Huan-xia,MA Hao,ZHAO Tu-qiang.Study on Optimization for RAPD Reaction System and Genetic Relationship in Beta vulgaris[J].Journal of Xinjiang Agricultural University,2010,33(1):1-6. | |
| Authors: | LA Ping LUO Shu-ping ZHANG Jian-xin GUO Chang-kui LIU Huan-xia MA Hao ZHAO Tu-qiang |
| Institution: | 1. College of Agronomy, Xinjiang Agricultural University, Urumqi 830052, China; 2. Shihhotze Institute of Beta Vulgaris, Shihhotze 832011, China ; 3. Nanjing Agricultural Agricultural University, Nanjing 210095, China) |
| Abstract: | In this study,orthogonal design was used to optimize RAPD amplification system on sugar beet of five factors： Mg2＋ , dNTP, primers, Taq DNA polymerase and template in five levels, respectively. A suitable RAPD reaction system was established, namely, 25 μL reaction system containing 1 × PCR buffer,1.0 mmol/L Mg2＋ ,0.25 mmol/L dNTP,0. 4 μumol/L primer,25 ng DNA template and 1U Taq DNA polymerase. On the basis of this optimal reaction system, the genetic relationship of 38 sugar beet cuttivars was studied. It was revealed that the polymorphism of RAPD mark in the 38 eultivars was very high and the variance was large. The result of clustering also showed that the genetic distance of 38 sugar beet culti- vars was ranging from 0.14 to 0.72, and could be classified into two groups when the genetic distance was 0.50, but when the genetic distance was 0.40; the second group could be classified into six subgroups. The result showed that this optimal reaction adapted to the RAPD reaction for identifying genetic relation- ships of sugar beets,wiich would provide important theoretical evidences for sugar beet breeding work. |
| Keywords: | RAPD |
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