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| 人c-myc乳腺定位表达转基因小鼠的建立 | |
| 引用本文： | 赵君,张守峰,刘彬,任文陟,宇丽,乔贵林,扈荣良,殷震.人c-myc乳腺定位表达转基因小鼠的建立[J].中国兽医学报,2002,22(6):587-589. |
| 作者姓名： | 赵君 张守峰 刘彬 任文陟 宇丽 乔贵林 扈荣良 殷震 |
| 作者单位： | 1. 解放军军需大学军事兽医研究所,解放军基因工程重点实验室,吉林,长春,130062 2. 湖南大学,生命科学院,湖南,长沙,410000 3. 暨南大学,生命科学院,广东,广州,510000 |
| 基金项目： | 国家自然科学基金资助项目 ( 9710 0 10 9) |
| 摘 要： | 以含有 1.6 kb的小鼠乳清酸蛋白 (WAP)上游调序列的 p CAT- WAP和含有人 c- myc c DNA的 p WM为原始质粒 ,构建了 WAP启动子调控下的 c- myc乳腺定位表达载体 p WCS。载体用 Bgl Bam H 酶切 ,回收 3.5 kb的基因片段 WAP- c- myc- SV40 Poly A,通过显微注射方法导入 C5 7BL/ 6 J× DBA/ 2 JF1 代小鼠受精卵的雄原核内。共注射10 0 0枚卵 ,将存活的约 6 0 0枚卵分别移植至 2 9只假孕母鼠输卵管内 ,获得仔鼠 45只。PCR检测 ,阳性鼠 9只 ,South-ern blot检测 ,阳性鼠 3只 ,其中 1只母鼠 ,2只公鼠 ,在饲养过程中 ,1只母鼠意外死亡。对 2只转基因公鼠的 F1代PCR检测表明 ,仅 1只公鼠具有遗传性 ,在所生的 2 7只 F1代小鼠中有 13只为 PCR阳性。 |
| 关 键 词： | c-myc 转基因 小鼠 乳腺 定位表达 |
| 文章编号： | 1005-4545(2002)06-0587-03 |
| 修稿时间： | 2002年9月11日 |
Establishment of Transgenic Mice Expressing c-myc Gene in Mammary Gland |
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| ZHAO Jun ,ZHANG Shou-feng ,LIU Bin ,REN Wen-zhi ,YU Li ,QIAO Gui-lin ,HU Rong-liang ,YIN Zhen.Establishment of Transgenic Mice Expressing c-myc Gene in Mammary Gland[J].Chinese Journal of Veterinary Science,2002,22(6):587-589. | |
| Authors: | ZHAO Jun ZHANG Shou-feng LIU Bin REN Wen-zhi YU Li QIAO Gui-lin HU Rong-liang YIN Zhen |
| Institution: | ZHAO Jun 1,ZHANG Shou-feng 1,LIU Bin 2,REN Wen-zhi 1,YU Li 3,QIAO Gui-lin 1,HU Rong-liang 1*,YIN Zhen 1 |
| Abstract: | c-myc cDNA was cloned on the downstream of mouse WAP promoter to form a expression vector pWCS.Transgenic mice were established by microinjection of the 3.5 kb c-myc cDNA expression cassette purified from pWCS.600 ova were transplanted into twenty-nine pseudopregnant mouse.recipients.45 offspring were born from the ova mircroinjected.9 of them were proved to be gene integration positively by PCR,3 mice,1 male and 2 female,were further confirmed to be transgenic by Southern hybridization.The female mice died by accidently during its feeding.1 of the other 2 male mice could inherit the c-myc gene into its F1 generation proved by PCR.6 female of 35 mice in its F1 generation were comfirmed to be transgenic by PCR.They are at a stage of mutiple pregnancy and lactation. |
| Keywords: | c-myc transgenic mouse mammary gland targeting expression |
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