| 木薯苯丙氨酸解氨酶基因的克隆及其对低温胁迫的响应 |
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| 引用本文： | 吴远航,刘秦,刘攀道,郭鹏飞,李敏,蒋凌雁,罗丽娟.木薯苯丙氨酸解氨酶基因的克隆及其对低温胁迫的响应[J].热带作物学报,2019,40(3):483-489. |
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| 作者姓名： | 吴远航 刘秦 刘攀道 郭鹏飞 李敏 蒋凌雁 罗丽娟 |
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| 作者单位： | 1. 海南大学热带农林学院,海南海口 5702282. 中国热带农业科学院热带作物品种资源研究所,海南儋州 571737 |
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| 基金项目： | 国家自然科学基金项目(31660412) |
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| 摘 要： | 本研究从全基因组水平对木薯（Manihot esculenta Crantz）苯丙氨酸解氨酶编码基因（Phenylalanine ammonia-lyase,PAL）进行鉴定,并以木薯品种KU50为材料克隆了6个PAL基因,分析了低温胁迫（7 ℃）对叶片MePAL表达模式、PAL酶活性、总酚含量、类黄酮含量和总抗氧化能力的影响。结果表明：低温处理使木薯叶片迅速萎蔫卷曲,并伴随叶片PAL酶活性、总酚含量、类黄酮含量和总抗氧化能力的显著提高。Real-time PCR分析表明,在低温处理前,MePAL1和MePAL2的相对表达量分别为0.83和1.19,显著高于其他4个MePAL基因,低温处理后6个MePAL基因均不同程度受低温胁迫诱导增强表达,其中MePAL5上调最高,达50.5~142.5倍。本研究结果初步显示低温胁迫上调了木薯叶片MePAL的表达,促进了总酚和类黄酮的合成,提高了抗氧化损伤的能力。
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| 关 键 词： | 木薯 低温胁迫 苯丙氨酸解氨酶基因 类黄酮 抗氧化 |
| 收稿时间： | 2018-06-05 |
Cloning of Cassava Phenylalanine Ammonia Lyase Genes and Their Responses to Low Temperature Stress |
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| WU Yuanhang,LIU Qin,LIU Pandao,GUO Pengfei,LI Min,JIANG Lingyan,LUO Lijuan.Cloning of Cassava Phenylalanine Ammonia Lyase Genes and Their Responses to Low Temperature Stress[J].Chinese Journal of Tropical Crops,2019,40(3):483-489. |
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| Authors: | WU Yuanhang LIU Qin LIU Pandao GUO Pengfei LI Min JIANG Lingyan LUO Lijuan |
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| Institution: | 1. Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan 570228, China2. Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou, Hainan 571737, China |
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| Abstract: | Cassava phenylalanine ammonia-lyase encoding genes (MePALs) were identified from the whole genome sequence data. Six MePALs genes, MePAL1-6, were subsequently cloned by RT-PCR from the leaves of the cultivar KU50 and their expression patterns were analyzed, together with PAL activity, total phenolic content, flavonoid content and total antioxidant capacity, in the leaves under low temperature stress treatment (7 ℃). It was found that low temperature treatment resulted in rapid leaf wilting and curling and correspondingly a significant increase in PAL activity, total phenol content, flavonoid content and total antioxidant capacity in the leaves. Real-time PCR analysis showed that the relative expression level of MePAL1 and MePAL2 was 0.83 and 1.19 respectively before low temperature treatment, which was significantly higher than the other 4 MePALs. Low temperature treatment increased the expression of all the six MePAL genes and the up-regulation of MePAL5 was the highest (increased from 50.5 to 142.5 folds). Our preliminary results showed that low temperature treatment increased the expression of MePALs, promoted the synthesis of total phenols and flavonoids, and enhanced anti-oxidative capacities in cassava leaves. |
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| Keywords: | cassava low temperature stress phenylalanine ammonia-lyase gene flavonoids antioxidant |
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