| 外源海藻糖调节西瓜细胞渗透胁迫抗性的研究 |
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| 引用本文： | 孙梦利,王世豪,徐子健,江雪飞,孙化鹏,乔飞,丛汉卿,于平.外源海藻糖调节西瓜细胞渗透胁迫抗性的研究[J].热带作物学报,2019,40(2):269-274. |
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| 作者姓名： | 孙梦利 王世豪 徐子健 江雪飞 孙化鹏 乔飞 丛汉卿 于平 |
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| 作者单位： | 1. 海南大学园艺学院/海南省热带生物资源可持续利用重点实验室,海南海口 5702282. 中国热带农业科学院热带作物品种资源研究所/农业农村部华南作物基因资源与种质创制重点开放实验室,海南儋州 5717373. 黑龙江八一农垦大学农学院,黑龙江大庆 613319 |
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| 基金项目： | 国家自然科学基金项目(31760595);海口市重点科技计划项目(2017045);海南省教育厅高等学校科学研究项目(Hnky2017-6) |
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| 摘 要： | 为探究外源海藻糖对渗透胁迫下西瓜细胞生长的影响,本研究以西瓜悬浮培养细胞为试材,测定外源海藻糖预处理后,渗透胁迫对西瓜悬浮培养细胞生长量、细胞外pH、细胞内ROS（活性氧簇）相对含量和微管骨架的影响。结果表明：渗透胁迫下西瓜细胞的生长量明显受到抑制,并且渗透胁迫可诱导西瓜悬浮培养细胞质外体碱化,ROS迸发,细胞微管骨架发生解聚;外源添加海藻糖可在一定程度上缓解渗透胁迫对西瓜悬浮细胞生长量的抑制作用,调节pH、ROS表达水平、并维持微管骨架结构的完整性。以上研究结果表明渗透胁迫下,海藻糖对西瓜细胞具有维持细胞生长、保护亚细胞结构并调节抗性反应的功能。
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| 关 键 词： | 西瓜 渗透胁迫 海藻糖 活性氧 微管骨架 |
| 收稿时间： | 2018-07-03 |
Exogenous Trehalose Modulating the Tolerance of Osmotic Stress in Watermelon Cells |
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| SUN Mengli,WANG Shihao,XU Zijian,JIANG Xuefei,SUN Huapeng,QIAO Fei,CONG Hanqing,YU Ping.Exogenous Trehalose Modulating the Tolerance of Osmotic Stress in Watermelon Cells[J].Chinese Journal of Tropical Crops,2019,40(2):269-274. |
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| Authors: | SUN Mengli WANG Shihao XU Zijian JIANG Xuefei SUN Huapeng QIAO Fei CONG Hanqing YU Ping |
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| Abstract: | In order to explore the effect of exogenous trehalose on watermelon cells under osmotic stress, watermelon suspension culture cells were used as the materials in this study. The changes of cell growth, extracellular pH, intracellular ROS content, and microtubule skeletons under mannitol stress in presence of exogenous trehalose were measured. The results showed that osmotic stress could inhibit cell growth, induce extracellular alkalization, ROS bursting, and counteract the depolymerization of microtubular cytoskeleton. However, exogenous trehalose could alleviate the inhibitory effect on cell growth caused by osmotic stress, suppress the expression of extracellular alkalization and ROS, and maintain the integrity of the microtubular cytoskeleton. The above results indicated that trehalose had protective effects on watermelon cell growth by maintaining subcellular structure and tuning stress related signals against osmotic stress. |
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| Keywords: | watermelon osmotic stress trehalose ROS microtubular cytoskeleton |
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