| 植物组织培养过程中的DNA甲基化研究进展 |
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| 引用本文： | 石鹏,王永,金龙飞,张大鹏,赵志浩,曹红星,雷新涛.植物组织培养过程中的DNA甲基化研究进展[J].热带作物学报,2019,40(1):199-207. |
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| 作者姓名： | 石鹏 王永 金龙飞 张大鹏 赵志浩 曹红星 雷新涛 |
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| 作者单位： | 中国热带农业科学院椰子研究所/海南省热带油料作物生物学重点实验室,海南文昌 571339 |
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| 基金项目： | 中国热带农业科学院基本科研业务费专项资金(17CXTD-13);农作物种质资源保护与利用项目(2016NWB052) |
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| 摘 要： | 组织培养是植物无性繁殖和遗传转化的重要技术基础。组织培养包括外植体脱分化、愈伤组织形成、再分化等过程,最后形成新的植株。在植物组织培养过程中,DNA甲基化模式不断发生变化,表明植物细胞发生了大量的表观遗传重编程事件。一般来说,DNA甲基化可以促进或加快组织培养过程,对于许多很难进行组织培养和再生的植物来说非常重要。本文主要总结了植物组织培养过程中DNA甲基化模式的变化规律和分子机制,以及新的研究手段和思路,旨在为开展植物组织培养过程中的DNA甲基化研究提供帮助。
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| 关 键 词： | 植物 组织培养 DNA甲基化 |
| 收稿时间： | 2018-07-13 |
Research Progress on DNA Methylation During Plant Tissue Culture |
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| SHI Peng,WANG Yong,JIN Longfei,ZHANG Dapeng,ZHAO Zhihao,CAO Hongxing,LEI Xintao.Research Progress on DNA Methylation During Plant Tissue Culture[J].Chinese Journal of Tropical Crops,2019,40(1):199-207. |
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| Authors: | SHI Peng WANG Yong JIN Longfei ZHANG Dapeng ZHAO Zhihao CAO Hongxing LEI Xintao |
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| Institution: | Coconut Research Institute, Chinese Academy of Tropical Agricultural Sciences / Hainan Key Biological Laboratory of Tropical Oil Crops, Wenchang, Hainan 571339, China |
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| Abstract: | Tissue culture is the important technical basis for plant asexual propagation and genetic transformation. Tissue culture includes explants dedifferentiation, callus formation, callus redifferentiation and plantlets formation. DNA methylation pattern is constantly changing during tissue culture, which means many epigenetic reprogramming events occur in cells. Generally speaking, DNA methylation can promote or accelerate the tissue culture process, it’s very important for many plants that are difficult to regenerate. The paper mainly summarizes the pattern change and molecular mechanism of DNA methylation during plant tissue culture, as well as new methods and ideas. We hope the paper could help the study of DNA methylation in plant tissue culture. |
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| Keywords: | plant tissue culture DNA methylation |
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