| 内源H2O2对水稻种子萌发的影响 |
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| 引用本文： | 肖羽,冯红玉,陈惠萍.内源H2O2对水稻种子萌发的影响[J].热带作物学报,2019,40(2):247-253. |
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| 作者姓名： | 肖羽 冯红玉 陈惠萍 |
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| 作者单位： | 海南大学热带农林学院,海南海口 570228 |
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| 基金项目： | 国家自然科学基金项目(31560426) |
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| 摘 要： | 通过用不同浓度的烟酰胺腺嘌呤二核苷酸磷酸（nicotinamide adenine dinucleotide phosphate, NADPH）合成酶抑制剂碘二苯（diphenylene iodonium, DPI）及过氧化氢（hydrogen peroxide, H2O2）清除剂二甲基硫脲（dimethylthiourea, DMTU）分别培养水稻（Oryza sativa L.）种子,研究内源H2O2对种子萌发过程胚根、胚芽和胚根根尖活力等的影响。结果表明,DPI及DMTU培养的水稻种子其胚根生长和胚芽生长均受到抑制,尤其是DPI对胚根生长的抑制作用更为显著。DPI和DMTU对水稻种子萌发的影响均呈现出浓度效应,即浓度越高,抑制作用越强。其中,DPI对水稻种子萌发的抑制作用比DMTU的更为明显。此外,胚根根尖的超氧阴离子（superoxide anion, O 2-）和H2O2含量随DMTU浓度增大而减少,根尖细胞受损也越严重。由此推测,内源H2O2可能参与调控水稻种子萌发过程。
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| 关 键 词： | 内源H2O2 种子萌发 DPI DMTU |
| 收稿时间： | 2018-08-01 |
Effect of Endogenous H2O2 on the Germination of Rice Seeds |
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| XIAO Yu,FENG Hongyu,CHEN Huiping.Effect of Endogenous H2O2 on the Germination of Rice Seeds[J].Chinese Journal of Tropical Crops,2019,40(2):247-253. |
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| Authors: | XIAO Yu FENG Hongyu CHEN Huiping |
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| Institution: | Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan 570228, China |
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| Abstract: | To explore the effect of endogenous hydrogen peroxide (H2O2) on the growth of radicle and plumule, and the apical vitality in the radicle during germination, rice (Oryza sativa L.) seeds were cultured with different concentrations of nicotinamide adenine dinucleotide phosphate (NADPH) synthase inhibitor diphenylene iodonium (DPI) or H2O2 scavenger dimethylthiourea (DMTU). The results showed that the growth of radicle and plumule in the rice seeds cultured with both DMTU and DPI was inhibited, especially that with DPI. Higher concentration of DPI or DMTU resulted in stronger inhibitory effect on rice seed germination. The inhibitory effect of DPI on rice seed germination was more obvious than that of DMTU. In addition, the contents of superoxide anion (O 2-) and H2O2 decreased in the apical of radicle with the increase of DMTU concentration, and the number of damage cell increased. Therefore, It suggested that endogenous H2O2 may be involved in regulating the germination of rice seeds. |
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| Keywords: | endogenous H2O2 seed germination DPI DMTU |
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