| 贵港报春苣苔的叶片组培与植株再生 |
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| 引用本文： | 闫海霞,黄昌艳,张自斌,崔学强,邓杰玲,关世凯,卜朝阳.贵港报春苣苔的叶片组培与植株再生[J].热带作物学报,2019,40(1):98-106. |
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| 作者姓名： | 闫海霞 黄昌艳 张自斌 崔学强 邓杰玲 关世凯 卜朝阳 |
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| 作者单位： | 广西壮族自治区农业科学院花卉研究所,广西南宁 530007 |
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| 基金项目： | 广西创新驱动发展专项资金项目(桂科AA17204046-1);广西农业科学院项目(桂农科2018YT33,No. 2018NZ01) |
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| 摘 要： | 以贵港报春苣苔的叶片为外植体,研究不同培养基对其不定芽诱导和增殖、愈伤组织诱导与分化以及生根的影响。结果表明,外植体叶片以纵切为宜,不定芽诱导最适培养基为MS+6-BA 4.0 mg/L+IAA 1.5 mg/L,愈伤组织诱导最适培养基为MS+6-BA3.0～5.0 mg/L+2,4-D0.5～1.0 mg/L,不定芽诱导率以及愈伤组织诱导率均为100.00%;愈伤在MS+KT 1.0 mg/L+NAA 0.2 mg/L+potato 30 g/L+banana 30 g/L+apple 20 g/L+coconut juice 100 mL/L培养基上分化系数达12.64;不定芽在MS+ZT 1.0 mg/L+NAA 0.10 mg/L+potato 30 g/L+banana 30 g/L+apple 20 g/L+coconut juice 100 mL/L培养基的增殖系数为8.55;不定芽在3/4 MS+NAA 0.01～0.05 mg/L+活性炭1.0～3.0 g/L培养基上的生根率为100.00%。综上所述,叶片纵切后能通过不定芽途径以及愈伤组织途径建立贵港报春苣苔的组织培养技术体系,在该体系下不定芽增殖系数高、愈伤分化高,组培苗生根好。
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| 关 键 词： | 贵港报春苣苔 组织培养 再生体系 天然提取物 |
| 收稿时间： | 2018-05-16 |
Tissue Culture and Plant Regeneration of Leaves of Primulina guigangensis |
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| YAN Haixia,HUANG Changyan,ZHANG Zibin,CUI Xueqiang,DENG Jieling,GUAN Shikai,BU Zhaoyang.Tissue Culture and Plant Regeneration of Leaves of Primulina guigangensis[J].Chinese Journal of Tropical Crops,2019,40(1):98-106. |
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| Authors: | YAN Haixia HUANG Changyan ZHANG Zibin CUI Xueqiang DENG Jieling GUAN Shikai BU Zhaoyang |
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| Institution: | Flower Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi 530007, China |
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| Abstract: | The effects of different media on the primary induction, adventitious buds proliferation, callus differentiation and rooting culture were investigated using the leaves of P. guigangensis as the explants. The results showed that the rip cutting was the suitable method; the suitable medium for adventitious buds regeneration was MS+6-BA 4.0 mg/L+IAA 1.5 mg/L; the suitable medium for callus induction was MS+6-BA 3.0-5.0 mg/L+2,4-D 0.5-1.5 mg/L; both rate of adventitious buds induction and rate of callus induction were 100.00%; the suitable medium for callus differentiation was MS+KT 1.0 mg/L+NAA 0.2 mg/L+potato 30 g/L+banana 30 g/L+apple 20 g/L+coconut juice 100 mL/L, and the differentiation coefficient was 12.64; the suitable medium for adventitious buds proliferation was MS+ZT 1.0 mg/L+NAA 0.10 mg/L+potato 30 g/L+banana 30 g/L+apple 20 g/L+coconut juice 100 mL/L, and the multiplication coefficient was 8.55; the suitable medium for rooting culture was 3/4 MS+NAA 0.01-0.05 mg/L+active carbon 1.0-3.0 g/L and the rooting rate was 100%. It was concluded that the leaves rip cutting could be used to establish the tissue culture system of P. guigangensis by the regeneration methods of adventitious buds and callus which had high induction and proliferation rates of adventitious buds and good rooting growth. |
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| Keywords: | Primulina guigangensis tissue culture regeneration system natural extracts |
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