| 草甘膦对蚯蚓体内抗氧化酶和AChE酶活性的影响 |
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| 引用本文： | 王冰洁,姜蕾,潘波,刘迎,林勇.草甘膦对蚯蚓体内抗氧化酶和AChE酶活性的影响[J].热带作物学报,2019,40(9):1804-1809. |
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| 作者姓名： | 王冰洁 姜蕾 潘波 刘迎 林勇 |
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| 作者单位： | 1. 中国热带农业科学院环境与植物保护研究所,海南海口 5711012. 农业农村部热带作物病虫害综合治理重点实验室,海南海口 571101 |
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| 基金项目： | 海南省重点研发计划项目(ZDYF2018056);中国热带农业科学院基本科研业务费专项资金(1630042017018) |
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| 摘 要： | 蚯蚓是土壤健康的关键指示生物,为了更好地评价草甘膦对土壤无脊椎动物的影响,本研究采用土壤法测定草甘膦对蚯蚓的急性毒性;用不同浓度的草甘膦分别测定不同暴露时间下对蚯蚓超氧化物歧化酶、过氧化氢酶、过氧化物酶、谷胱甘肽S转移酶、乙酰胆碱酯酶活性的影响。结果显示,土壤法测定草甘膦对蚯蚓的LC50>1000 mg/kg;在草甘膦的影响下,随着暴露时间的延长,蚯蚓SOD酶活力有着先增高后下降的趋势,CAT酶活力呈现着先下降后升高的趋势,随着时间和浓度的增长POD酶活力在草甘膦的影响下有逐渐下降的趋势;相对于空白对照,处理组AChE酶活力在草甘膦暴露14 d时显著降低。研究结果将为合理评价和监测草甘膦造成的土壤污染水平提供科学依据。
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| 关 键 词： | 草甘膦 蚯蚓 急性毒性 酶活力 |
| 收稿时间： | 2019-03-19 |
Effect of Glyphosate on the Activities of Antioxidase Enzymes and Acetylcholinesterases in Vivo Earthworm |
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| WANG Bingjie,JIANG Lei,PAN Bo,LIU Ying,LIN Yong.Effect of Glyphosate on the Activities of Antioxidase Enzymes and Acetylcholinesterases in Vivo Earthworm[J].Chinese Journal of Tropical Crops,2019,40(9):1804-1809. |
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| Authors: | WANG Bingjie JIANG Lei PAN Bo LIU Ying LIN Yong |
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| Institution: | 1. Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China2. Key Laboratory of Integrated Pest Management of Tropical Crops, Ministry of Agriculture & Rural Affairs, Haikou, Hainan 571101, China; |
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| Abstract: | Earthworm (Eisenia fetida) is one of the key bio-indicators of soil health. To evaluate the effects of glyphosateon soil invertebrates, the acute toxicity of glyphosate to earthworms were determined by artificial soil test. Effects of different concentrations of glyphosate on the activities of superoxidedismutase (SOD), catalase (CAT), peroxidase (POD), glutathions S-transferase (GST) and acetylcholinesterase (AChE) in earthworms were measured at different exposure durations. The results of artificial soil test showed that the LC50 of glyphosate to earthworms was greater than 1000 mg/kg (7 d, 14 d), indicating that the acute toxicity of glyphosate was low to earthworms. With the increase of glyphosate concentrations and exposure time, the activity of SOD in earthworms increased first and then decreased; the activity of CAT in earthworms decreased first and then increased; and the activity of POD in earthworms kept decreasing. Compared to the control, the activity of AChE in earthworms exposed to glyphosate reduced significantly after 14 days. The results would provide a scientific basis for rational evaluation and monitoring of soil pollution level caused by glyphosate. |
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| Keywords: | glyphosate earthworm acute toxicity enzyme activity |
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