| 红树林来源真菌Xylaria sp. HNWSW-2异香豆素类次生代谢产物及其生物活性的研究 |
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| 引用本文： | 权云帆,王佩,王昊,袁靖喆,戴好富,梅文莉.红树林来源真菌Xylaria sp. HNWSW-2异香豆素类次生代谢产物及其生物活性的研究[J].热带作物学报,2019,40(8):1611-1617. |
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| 作者姓名： | 权云帆 王佩 王昊 袁靖喆 戴好富 梅文莉 |
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| 作者单位： | 1. 海南大学热带农林学院,海南海口 5702282. 中国热带农业科学院热带生物技术研究所/海南省黎药资源天然产物研究与利用重点实验室,海南海口 5711013. 海南医学院热带医学与检验医学院,海南海口 571199 |
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| 基金项目： | 国家自然科学基金青年科学基金项目(No.41506096);国家自然科学基金面上项目(No.41776093);农业农村部财政专项项目(No.NFZX2018) |
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| 摘 要： | 为了研究红树林来源真菌Xylaria sp. HNWSW-2的次生代谢产物及其生物活性,综合利用多种色谱技术对该菌株发酵产物进行分离纯化,结合波谱学与理化常数分析进行化合物结构鉴定,分别采用液体浸泡法和Ellman比色法对化合物的全齿复活线虫致死活性和乙酰胆碱酯酶抑制活性进行测试。从Xylaria sp. HNWSW-2发酵产物乙酸乙酯萃取物中分离鉴定了7个异香豆素类化合物,分别为 (S)-(+)-8-O-methylmellein (1),(3S,4S)-(+)-4-hydroxy-8-O- methylmellein (2),(3S,4R)-(+)-4-hydroxy-8-O-methylmellein (3),(3S,4S)-(+)-4-hydroxymellein (4),(3S,4R)-(+)-4- hydroxymellein (5),(3R,4R)-(-)-4-hydroxy-5-methylmellein (6)和(3R,4S)-(+)-4-hydroxy-5-methylmellein (7)。其中,化合物1具有较强全齿复活线虫致死活性,化合物1~3、6和7具有一定的乙酰胆碱酯酶抑制活性。本研究首次发现化合物(S)-(+)-8-O-methylmellein具有较强的抗线虫活性,为相关杀线虫药物的研发提供理论依据。
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| 关 键 词： | 红树林 真菌 次生代谢产物 异香豆素 生物活性 |
| 收稿时间： | 2019-01-09 |
Isocoumarin Derivatives from Mangrove-derived Fungus Xylaria sp. HNWSW-2 and the Biological Activities |
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| QUAN Yunfan,WANG Pei,WANG Hao,YUAN Jingzhe,DAI Haofu,MEI Wenli.Isocoumarin Derivatives from Mangrove-derived Fungus Xylaria sp. HNWSW-2 and the Biological Activities[J].Chinese Journal of Tropical Crops,2019,40(8):1611-1617. |
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| Authors: | QUAN Yunfan WANG Pei WANG Hao YUAN Jingzhe DAI Haofu MEI Wenli |
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| Institution: | 1. Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan 570228, China2. Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences / Hainan Key laboratory for Research and Development of Natural Products from Li Folk Medicine, Haikou, Hainan 571101, China3. School of Tropical and Laboratory Medicine, Hainan Medical University, Haikou, Hainan 571199, China |
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| Abstract: | In order to study the secondary metabolites of the mangrove-derived fungus Xylaria sp. HNWSW-2 and the biological activities, seven isocoumarin derivatives were isolated by various column chromatographic techniques. The chemical structures were identified as (S)-(+)-8-O-methylmellein (1), (3S,4S)-(+)-4-hydroxy-8-O-methylmellein (2), (3S,4R)-(+)-4-hydroxy-8-O-methylmellein (3), (3S,4S)-(+)-4-hydroxymellein (4), (3S,4R)-(+)-4-hydroxymellein (5), (3R,4R)-(-)-4-hydroxy-5-methylmellein (6) and (3R,4S)-(+)-4-hydroxy-5-methylmellein (7) based on a combined analysis of spectral data and physicochemical properties. In addition, all compounds were tested for the nematicidal activity against Panagrellus redivivus and acetylcholinesterase (AChE) inhibitory activity by liquid immersion method and Ellman colorimetric method, respectively. Among them, compound 1 exhibited nematicidal activity against P. redivivus. Compounds 1-3, 6 and 7 showed AChE inhibitory activity. The potential nematicidal activity of compound 1 would provide scientific evidences for its development of nematicide. |
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| Keywords: | mangrove fungus secondary metabolites isocoumarin derivatives bioactive activities |
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