| 昼夜温差处理下铁皮石斛原球茎松柏苷和紫丁香苷含量的测定 |
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| 引用本文： | 王丛巧,王培育,郭艳芳,鲁瑶,彭丽云,陈青青,林玉玲,赖钟雄.昼夜温差处理下铁皮石斛原球茎松柏苷和紫丁香苷含量的测定[J].热带作物学报,2019,40(2):261-268. |
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| 作者姓名： | 王丛巧 王培育 郭艳芳 鲁瑶 彭丽云 陈青青 林玉玲 赖钟雄 |
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| 作者单位： | 福建农林大学园艺植物生物工程研究所,福建福州 350002 |
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| 基金项目： | 福建省科技重大专项“福建特色园艺作物种质优异基因资源的挖掘与种质创新”(2015NZ0002-1);福建农林大学创新基金(CXZX 2017189) |
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| 摘 要： | 研究不同昼夜温差对铁皮石斛原球茎松柏苷、紫丁香苷含量的影响,为开发铁皮石斛原球茎作为药材提供研究基础。采用光照培养箱设置不同温差处理,采用超高效液相色谱法测定松柏苷、紫丁香苷含量变化。建立同时测定松柏苷和紫丁香苷的超高效液相色谱法,以ACQUITY UPLC BEH C18（2.1 mm×100 mm,1.7 μm）为分析柱;流动相：1%乙酸水溶液（A）和甲醇（C）。正交试验研究提取方法,结果表明用纯水提取,料液比1∶40,提取1 h（每0.5 h震荡一次）,2种物质提取率最高。采用此方法测定4个昼夜温差处理的铁皮石斛原球茎样品,结果表明,对照0 ℃温差松柏苷、紫丁香苷整体变化趋于平缓,8 ℃温差处理下,松柏苷和紫丁香苷在10 d的处理周期下都表现出含量增加,且明显优于其他处理。4 ℃温差处理两物质含量在第15天达到最高,12 ℃温差处理两物质含量前期高于对照处理,处理后期低于对照处理,且低于4 ℃、8 ℃温差处理。昼夜温差影响铁皮石斛原球茎松柏苷、紫丁香苷含量,一定范围内的温差呈现促进作用,温差过大则出现抑制作用。
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| 关 键 词： | 铁皮石斛 原球茎 松柏苷 紫丁香苷 超高效液相色谱 昼夜温差 |
| 收稿时间： | 2018-08-29 |
Determination of Coniferin and Syringin in Protocorms of Dendrobium officinale under Diurnal Temperature Difference |
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| WANG Congqiao,WANG Peiyu,GUO Yanfang,LU Yao,PENG Liyun,CHEN Qingqing,LIN Yuling,LAI Zhongxiong.Determination of Coniferin and Syringin in Protocorms of Dendrobium officinale under Diurnal Temperature Difference[J].Chinese Journal of Tropical Crops,2019,40(2):261-268. |
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| Authors: | WANG Congqiao WANG Peiyu GUO Yanfang LU Yao PENG Liyun CHEN Qingqing LIN Yuling LAI Zhongxiong |
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| Institution: | Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China |
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| Abstract: | To understand the effects of temperature difference between day and night on the contents of coniferin and syringin in the protocorm of Dendrobium officinale, which would provide a the research basis for the development of protocorm of D. officinale as a medicinal material in large scale production. The fast method based on ultra-high performance liquid chromatography (UPLC) for the analysis of coniferin and syringin, the chromatographic separation was carried on an ACQUITY UPLC BEH C18 (2.1 mm × 100 mm, 1.7 μm) in gradient elution with a mobile phase of acetic acid aqueous solution (1%, w/w) (A) and methanol (C). The extraction method of an orthogonal test was designed, and the results showed that the sample extracted with pure water, extraction ratio 1:40, and the extraction time 1 h (vibrate every half an hour) had the highest extraction rate of the two substances. The proposed method was applied to the determination of four samples of D. officinale protocorm in temperature difference treatment. The results showed that the whole change of syringin and coniferin tended to be gental at 0 ℃. The contents of coniferin and syringin increased in the 10 d treatment with temperature difference 8 ℃ between day and night, and they were obviously superior to those of other treatments. The content of the two substances reached the highest on the 15 d treatment with temperature difference 4 ℃ between day and night. The contents of the two substances in the temperature difference 12 ℃ treatment were higher in the early stage than that in the control treatment, and were lower in the latter stage than that in the control treatment, and were also lower than that in the temperature difference 8 ℃ and 4 ℃ treatment. Moderate temperature difference appears to promote the effect, while big temperature difference lowers the effect. |
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| Keywords: | Dendrobium officinale protocorn coniferin syringin ultra high performance liquid chromatography temperature difference between day and night |
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