| 分散固相萃取法测定黄瓜中的喹啉铜残留量 |
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| 引用本文： | 陈思宇,王明月,林冰,吕岱竹.分散固相萃取法测定黄瓜中的喹啉铜残留量[J].热带作物学报,2019,40(7):1449-1454. |
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| 作者姓名： | 陈思宇 王明月 林冰 吕岱竹 |
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| 作者单位： | 1. 华中农业大学资源与环境学院,湖北武汉 4300702. 中国热带农业科学院分析测试中心,海南海口 571101 |
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| 基金项目： | 国家重点研发计划(2016YFD0201203) |
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| 摘 要： | 建立了分散固相萃取（d-SPE）-高效液相色谱（HPLC）分析测定黄瓜中喹啉铜残留量的方法。样品采用乙腈和0.1 mol/L盐酸混合溶液提取,C18填料净化,高效液相色谱仪分离,紫外检测器检测。其色谱条件为：色谱柱SunFireC18（4.6×150 mm,5 μm）,流动相为十二烷基硫酸钠磷酸盐缓冲液-乙腈（65∶35）体系,流速0.6 mL/min,检测波长250 nm。结果表明：在0.02、0.2、2 mg/kg三个添加水平下,喹啉铜回收率在92.3%~107.7%之间,相对标准偏差（RSD）在2.0%~7.7%之间。该方法操作简单、灵敏度和准确度高,能够用于黄瓜中喹啉铜的残留分析。
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| 关 键 词： | 黄瓜 喹啉铜 分散固相萃取 高效液相色谱法 |
| 收稿时间： | 2018-07-26 |
Determination of Oxine-Copper Residue in Cucumis sativus L. by Dispersive Solid Phase Extraction |
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| CHEN Siyu,WANG Mingyue,LIN Bing,LV Daizhu.Determination of Oxine-Copper Residue in Cucumis sativus L. by Dispersive Solid Phase Extraction[J].Chinese Journal of Tropical Crops,2019,40(7):1449-1454. |
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| Authors: | CHEN Siyu WANG Mingyue LIN Bing LV Daizhu |
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| Institution: | 1. College of Resources and Environment, Huazhong Agricultural University, Wuhan, Hubei 430070, China2. Analysis and Testing Center, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China |
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| Abstract: | A high performance liquid chromatography (HPLC) coupled with the dispersive solid phase extraction (d-SPE) method for the determination of oxine-copper residue in cucumber was established. The sample was extracted by acetonitrile and hydrochloric acid (0.1 mol/L). The extract was purified by C18. The analysis was carried out by phosphate buffer saline (PBS) and sodium dodecyl sulfate (SDS) -acetonitrile (65∶35) at a flow rate of 0.6 mL/min on SunFireC18 (4.6×150 mm, 5 μm) column, and detected by HPLC with UV detector at a ultraviolet absorption wavelength of 250 nm. The recovery was in 92.3%-107.7% with relative standard deviation (RSD) of 2.0%-7.7% at spiked levels of 0.02, 0.2 and 2 mg/kg. The method is simple, sensitive and accurate, and could be used in the determination of oxine-copper residue in cucumber. |
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| Keywords: | Cucumis sativus L oxine-copper dispersive solid phase extraction high performance liquid chromatography |
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