| 锰掺杂的碳点作为纳米模拟酶用于比色检测毒死蜱 |
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| 引用本文： | 白秋月,杨春亮,林丽云,叶剑芝.锰掺杂的碳点作为纳米模拟酶用于比色检测毒死蜱[J].热带作物学报,2019,40(6):1195-1204. |
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| 作者姓名： | 白秋月 杨春亮 林丽云 叶剑芝 |
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| 作者单位： | 1. 华中农业大学,湖北武汉 4300702. 中国热带农业科学院农产品加工研究所,广东湛江 524001 |
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| 基金项目： | 海南省自然科学基金青年基金项目(219QN290);农业农村部财政专项农产品质量安全监管风险评估项目(GJFP2018011);中国热带农业科学院基本科研业务费专项资金项目(1630122017020) |
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| 摘 要： | 以碳酸锰、脲、柠檬酸、双氧水为原料,采用微波加热法合成具有纳米模拟酶催化活性的锰掺杂碳点(Mn-CDs)。Mn-CDs可催化3,3’,5,5’-四甲基联苯胺(TMB)产生蓝色的ox-TMB。乙酰胆碱酯酶(AChE)催化底物乙酰硫代胆碱(ATCh)生成的硫代胆碱(TCh),还原所生成的ox-TMB使溶液蓝色褪去。有机磷类农药能有效抑制AChE的活性,使TCh的生成量减少,溶液的蓝色变深。根据吸光度的变化可以定量检测有机磷农药含量,由溶液颜色的深浅可以构建毒死蜱的可视化半定量检测方法。本研究表征了Mn-CDs的表面结构及微观形貌,以有机磷类农药主要品种毒死蜱作为分析模型,初步探讨了比色法检测毒死蜱的原理;考察了毒死蜱检测的最优条件,检测的线性范围是0~3.5μg/mL,检测限为0.013μg/mL。将该检测方法用于苹果实际样品中毒死蜱的测定,回收率为95.2%~102.8%,表明该方法有望应用于实际样品中有机磷的高灵敏测定。
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| 关 键 词： | 掺杂碳点 纳米模拟酶 毒死蜱 比色检测 |
| 收稿时间： | 2018-12-20 |
Mn Doped Carbon Dots as Nano-mimetic Enzyme for the Colorimetric Detection of Chlorpyrifos |
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| BAI Qiuyue,YANG Chunliang,LIN Liyun,YE Jianzhi.Mn Doped Carbon Dots as Nano-mimetic Enzyme for the Colorimetric Detection of Chlorpyrifos[J].Chinese Journal of Tropical Crops,2019,40(6):1195-1204. |
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| Authors: | BAI Qiuyue YANG Chunliang LIN Liyun YE Jianzhi |
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| Institution: | 1. Huazhong Agricultural University, Wuhan, Hubei 430070, China2. Agricultural Products Processing Research Institute , Chinese Academy of Tropical Agricultural Sciences, Zhanjiang, Guangdong 524001, China |
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| Abstract: | Manganese-doped carbon dots (Mn-CDs) with nano-simulated enzyme catalytic activity were synthesized by citric acid, urea, hydrogen peroxide and manganese carbonate. Mn-CDs catalyze the production of blue ox-TMB by 3,3°,5,5°- tetramethylbenzidine (TMB). Acetylcholinesterase (AChE) catalyzes the thiocholine (TCh) produced by the substrate acetylthiocholine (ATCh), and the resulting ox-TMB reduces the blue color of the solution. Organophosphorus pesticide can effectively inhibit the activity of AChE, reduce the production of TCh, and darken the blue of the solution. A visual detection method for organophosphorus pesticide can be constructed according to the depth of the solution color. The work described the surface structure and micromorphology of Mn-CDs. Utilized chlorpyrifos as an analytical model, which is the main species of organophosphorus pesticides. The principle of colorimetric detection of chlorpyrifos was discussed. The conditions for the detection of chlorpyrifos were investigated. The linear range of detection was 0-3.5 μg/mL and the detection limit was 0.013 μg/mL. The detection method was applied to the determination of chlorpyrifos in apple samples, and the recovery rate ranged from 95.2% to 102.8%, indicating that the method is expected to be applied to the highly sensitive determination of organic phosphorus in actual samples. |
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| Keywords: | doped carbon dots nano-mimetic enzyme chlorpyrifos colorimetric detection |
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