| 盐胁迫对扁蓿豆幼苗脯氨酸积累及其代谢关键酶活性的影响 |
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| 引用本文： | 钟华,董洁,董宽虎.盐胁迫对扁蓿豆幼苗脯氨酸积累及其代谢关键酶活性的影响[J].草业学报,2018,27(4):189-194. |
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| 作者姓名： | 钟华 董洁 董宽虎 |
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| 作者单位： | 山西农业大学动物科技学院,山西太谷,030801;北京市科学技术情报研究所,北京,100044 |
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| 基金项目： | “十一五”国家科技支撑计划课题(2007BAD56B01)，山西省科技攻关项目(20120311011-1) |
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| 摘 要： | 以野生扁蓿豆为试验材料,采用盆栽试验方法研究不同盐(NaCl、Na2CO3)的不同浓度(0、50、100、200、300、400、500 mmol·L-1)胁迫对扁蓿豆苗期脯氨酸积累及其代谢途径的关键酶活性的影响。结果表明,在不同盐胁迫下,扁蓿豆脯氨酸(Pro)含量、吡咯啉-5-羧酸合成酶(P5CS)活性、鸟氨酸-δ氨基转移酶(δ-OAT)活性均随盐浓度的升高而逐渐增加,而脯氨酸脱氢酶(ProDH)活性随胁迫浓度的升高而降低。扁蓿豆幼苗主动积累Pro是缓解其NaCl和Na2CO3胁迫的原因,并由Glu途径和Orn途径协同作用于Pro积累,但以Orn途径为主。试验中,扁蓿豆所承受的NaCl和Na2CO3浓度不宜超过400 mmol·L-1。
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| 关 键 词： | 扁蓿豆 盐胁迫 脯氨酸 吡咯啉-5-羧酸合成酶 鸟氨酸-δ氨基转移酶 脯氨酸脱氢酶 |
| 收稿时间： | 2017-11-01 |
| 修稿时间： | 2017-12-04 |
Effect of salt stress on proline accumulation and the activities of the key enzymes involved in proline metabolism in Medicago ruthenica seedlings |
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| ZHONG Hua,DONG Jie,DONG Kuan-hu.Effect of salt stress on proline accumulation and the activities of the key enzymes involved in proline metabolism in Medicago ruthenica seedlings[J].Acta Prataculturae Sinica,2018,27(4):189-194. |
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| Authors: | ZHONG Hua DONG Jie DONG Kuan-hu |
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| Institution: | 1.College of Animal Science and Veterinary Medicine, Shanxi Agriculture University, Taigu 030801, China;2.Beijing Science and Technology Information Institute, Beijing 100044, China |
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| Abstract: | In order to study the effects of salt stress on proline accumulation and the activities of the key enzymes involved in proline metabolism a pot experiment was conducted using wild Medicago ruthenica stressed under different NaCl and Na2COa solutions;0,50,100,200,300,400 and 500 mmol · L-1.The result showed that,under salt stress,proline content and P5CS and δ-OAT activity gradually increased while ProDH activity declined with increasing salt concentration.The initial accumulation of proline was a major reason for the reduced damage from NaCl and Na2CO3 stress in M.ruthenica seedlings.The Glu and Orn pathways were synergistic to the accumulation of proline,with the Orn pathway playing a leading role.This study suggests that the concentration of NaCl and Na2CO3 should not exceed 400 mmol · L 1 for M.ruthenica. |
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| Keywords: | Medicago ruthenica salt stress proline pyrroline-5-carboxylate synthetase ornithine-oxo-acid transaminase proline dehydrogenase |
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