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植物与土壤中醇类化合物对高寒草甸退化指示作用初探
引用本文:段中华,全小龙,乔有明,史惠兰,裴海昆,郑元铭.植物与土壤中醇类化合物对高寒草甸退化指示作用初探[J].草业科学,2018,35(5):969-977.
作者姓名:段中华  全小龙  乔有明  史惠兰  裴海昆  郑元铭
作者单位:三江源生态和高原农牧业国家重点实验室,青海 西宁810016;青海大学生态环境工程学院,青海 西宁810016;青海大学生态环境工程学院,青海 西宁,810016;三江源生态和高原农牧业国家重点实验室,青海 西宁,810016
基金项目:国家自然科学基金(31760691、31260573),国家重点研发计划(2017YFC0504803),国家国际科技合作专项项目(2015DFG31870),教育部美大项目(2016)
摘    要:醇类化合物是一类重要的生物标记物,被广泛用于土壤及沉积物中有机物质的溯源。为探究醇类化合物对高寒草甸退化指示作用规律,采用野外调查与气相色谱质谱联用(GC-MS)分析方法,对高寒草甸植物与土壤中醇类进行测定和分析。结果表明,高寒草甸植物中醇种类数为2~21,碳数为4~37,不饱和醇种类数高于饱和醇。退化土壤中检出9-甲基-10-顺式十五烯-1-醇、2-亚甲基-5α-胆甾烷-3β-醇、1-三十七烷醇,而在未退化土壤中未检出。高寒草甸中植物和土壤醇的种类大小关系为退化植物退化土壤未退化植物未退化土壤,植物和土壤中相同的醇有很强的相关性(R2=0.871)。植物和土壤醇类的分布特征及相关性分析表明,醇类化合物可以用作标记物来指示高寒草甸退化。

关 键 词:高寒草甸  植物  土壤  醇类  GC-MS  生物标记物  退化

Alcohols in plants and soil as indicators of alpine meadow degradation
Duan Zhong-hua,Quan Xiao-long,Qiao You-ming,Shi Hui-lan,Pei Hai-kun,Zheng Yuan-ming.Alcohols in plants and soil as indicators of alpine meadow degradation[J].Pratacultural Science,2018,35(5):969-977.
Authors:Duan Zhong-hua  Quan Xiao-long  Qiao You-ming  Shi Hui-lan  Pei Hai-kun  Zheng Yuan-ming
Abstract:Alcohols are an important class of biomarker and are widely used to trace the source of organic matter in soil and sediments.The present study was conducted to investigate the alcohol compositions of typical alpine meadows.The samples of alpine meadow plants and soil were collected,and gas chromatography-mass spec-trometry(GC-MS)analyses were carried out.The results showed that the range of alcohol types in the alpine meadow was from 2 to 21,the carbon number was from 4 to 37,and the number of unsaturated alcohols was higher than that of saturated alcohols.9-Methyl-Z-10-pentadecen-1-ol,(3,5)-2-methylene-Cholestan-3-ol,and 1-Heptatriacotanol were not detected in the non-degraded soil,but were found in the degraded soil.The species of plant and soil alcohols in alpine meadow were as follows:degraded plants > degraded soil > non-degraded plants > non-degraded soil.There was a strong correlation between the alcohols in the plants and those found in the soil(R2=0.871).The distribution characteristics and correlation analysis of plants and soil alcohols showed that alcohols could be used as markers to indicate the degradation of alpine meadow.
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