| 铁线莲‘Blekitny Aniol’组织培养及再生体系建立 |
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| 引用本文： | 黄鑫,张彦妮.铁线莲‘Blekitny Aniol’组织培养及再生体系建立[J].草业科学,2018,35(3):542-550. |
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| 作者姓名： | 黄鑫 张彦妮 |
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| 作者单位： | 东北林业大学园林学院,黑龙江哈尔滨,150040;东北林业大学园林学院,黑龙江哈尔滨,150040 |
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| 摘 要： | 对铁线莲(Clematis florida‘Blekitny Aniol’)的带芽茎段进行诱导产生无菌苗,并对其叶片和茎段进行愈伤组织诱导成苗的分化研究,成功建立了铁线莲组织培养再生体系。结果表明,带芽茎段诱导腋芽最佳培养基为MS+1mg·L~(-1)6-BA+0.05 mg·L~(-1)NAA,腋芽萌发率为100.0%;茎段诱导愈伤最适宜培养基为MS+2 mg·L~(-1)6-BA+0.01mg·L~(-1)NAA,诱导率为78.3%;叶片诱导愈伤组织最适宜的培养基为MS+1 mg·L~(-1)6-BA+0.1 mg·L~(-1)NAA,诱导率为81.7%;愈伤组织分化的最佳培养基为1/2MS+1 mg·L~(-1)6-BA+0.05 mg·L~(-1)NAA,分化率可达25.0%;最适宜不定芽的增殖培养基为1/2MS+3 mg·L~(-1)6-BA+0.1 mg·L~(-1)NAA,增殖倍数为4.94;筛选出最优的生根培养基组合为1/2MS+0.05 mg·L~(-1)NAA,生根率为70.2%。本研究将为铁线莲的推广应用提供参考,也为其他铁线莲属植物引种和开发利用奠定了基础。
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| 关 键 词： | 铁线莲 愈伤组织 不定芽 诱导率 分化率 再生 |
In vitro tissue culture and plant regeneration of Clematis florida 'Blekitny Aniol' |
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| Huang Xin,Zhang Yan-ni.In vitro tissue culture and plant regeneration of Clematis florida 'Blekitny Aniol'[J].Pratacultural Science,2018,35(3):542-550. |
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| Authors: | Huang Xin Zhang Yan-ni |
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| Abstract: | In this study,aseptic Clematis florida 'Blekitny Aniol' seedlings were obtained from stem segments with axillary buds,and callus induction and differentiation of leaves and stem segments were analyzed.The regeneration system of 'Blekitny Aniol' was established.The results showed that the optimal medium for the induction of axillary buds from the stem segments with axillary buds was MS+1 mg· L-16-BA+0.05 mg· L-1 NAA,and the germination rate of axillary buds was 100.0％.The optimal medium for callus induction from stem segments was MS+2 mg · L-16 BA+0.01 mg· L-1NAA with a callus induction rate of 78.3％.The optimal medium for callus induction from leaf blades was MS+1 mg· L-1 6-BA+0.1 mg · L-1NAA with the highest callus induction rate of 81.7％.The optimal medium for callus differentiation was 1/2MS+1 mg · L-1 6-BA+0.05 mg · L-1 NAA,and the callus differentiation rate was 25.0％.The optimal medium for adventitious budproliferation was 1/2MS+3 mg· L-16-BA+0.1 mg · L-1NAA,and the highest proliferation times was 4.94.Rooting rate of regenerated plants reached up to 70.2％ on half-strength MS medium supplemented with 0.05 mg· L-1NAA. |
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