| N+注入诱导突变乳酸菌筛选及对苜蓿青贮品质的影响 |
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| 引用本文： | 杨逢源,王雁萍,赵珊珊,郭琳娜,曹丹.N+注入诱导突变乳酸菌筛选及对苜蓿青贮品质的影响[J].草业科学,2018,35(1):208-213. |
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| 作者姓名： | 杨逢源 王雁萍 赵珊珊 郭琳娜 曹丹 |
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| 作者单位： | 郑州大学河南省离子束生物工程重点实验室,河南 郑州,450052;郑州大学河南省离子束生物工程重点实验室,河南 郑州,450052;郑州大学河南省离子束生物工程重点实验室,河南 郑州,450052;郑州大学河南省离子束生物工程重点实验室,河南 郑州,450052;郑州大学河南省离子束生物工程重点实验室,河南 郑州,450052 |
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| 基金项目： | 河南省重点科技攻关项目，河南省基础与前沿技术研究项目，郑州大学大学生创新创业训练计划项目 |
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| 摘 要： | 可溶性糖含量低影响乳酸菌添加剂产酸是影响苜蓿(Medicago sativa)青贮品质的重要因素之一。本研究采用N~+注入诱变的方法提高乳酸菌分解纤维素性能,以增强其在苜蓿青贮中的产酸能力。对具有良好抑菌活性及产酸能力的出发菌株植物乳杆菌ZZU A345进行N~+注入诱变,筛选出对羧甲基纤维素钠具有较好分解作用的突变菌株zw2-21和zw3-57,两株突变菌株酸化苜蓿粉的能力相较ZZU A345有显著(P0.05)提高,纤维素内切酶的活性亦高于ZZU A345,但没有显著差异(P0.05);苜蓿青贮试验结果显示,青贮65 d,添加zw2-21的处理组p H降至4.83,显著低于未添加乳酸菌的对照组(p H 5.5),也低于添加ZZU A345的处理组(p H 5.08),但没有显著差异;添加zw2-21的处理组乳酸含量达到1.22%,比添加ZZU A345的处理组提高了一倍。结果表明,N~+注入诱变技术在提高乳酸菌分解纤维素性能及苜蓿青贮高效产酸乳酸菌菌种选育方面具有潜在应用价值。
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| 关 键 词： | 植物乳杆菌 物理诱变 纤维降解 发酵产酸 CMC酶活 苜蓿青贮 有机酸 |
Screening of lactic acid bacteria after N+implantation and its effects on alfalfa silage quality |
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| Yang Feng-yuan,Wang Yan-ping,Zhao Shan-shan,Guo Lin-na,Cao Dan.Screening of lactic acid bacteria after N+implantation and its effects on alfalfa silage quality[J].Pratacultural Science,2018,35(1):208-213. |
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| Authors: | Yang Feng-yuan Wang Yan-ping Zhao Shan-shan Guo Lin-na Cao Dan |
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| Abstract: | Low soluble sugar content of alfalfa (Medicago sativa),which effects the acid production of lactic acid bacteria (LAB)additives,is one of the important factors that influences its silage quality.In this study, cellulolytic activities of lactic acid bacteria were enhanced by N+implantation to improve their acid production capabilities in alfalfa ensiling.Lactobacillus plantarumstrain ZZU A345 with high antimicrobial activity and good acid production ability was treated with a N+ion beam and the mutant strains zw2-2 1 and zw3-5 7 were screened out with good degradation of sodium carboxymethyl cellulose.The activity levels for alfalfa meal acidi-fication of the two strains was significantly higher (P<0.05)than that of ZZU A345,and the activity of cellu-lase endonuclease was also higher than that of ZZU A345,but no significant difference was found.Results for 65 d alfalfa ensiling showed that the pH of the treatment with zw2-21 decreased to 4.83,which was significant-ly lower (P<0.05)than that of the blank control group with no LAB additive(pH 5.5).It was also lower than that of the treatment with ZZU A345 (pH 5.08),but there was no significant difference.Content of lactic acid in the treatment with zw2-21 reached 1.22%,which was one time more than the content in the treatment with ZZU A345.We concluded that N+implantation has potential application for improving the performance of LAB in cellulose degradation and acid production in alfalfa ensiling. |
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