Improvement of Grain Shape and Fragrance by Using CRISPR/Cas9 System |
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| Authors: | XU Shanbin ZHENG Hongliang LIU Lifeng BU Qingyun Li Xiufeng ZOU Detang |
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| Institution: | 1.Key Laboratory of Germplasm Enhancement, Physiology and Ecology of Food Crops in Cold Region, Ministry of Education, Northeast Agricultural University, Harbin 150030, China;2.Northeast Institute of Geography and Agroecology, Key Laboratory of Soybean Molecular Design Breeding, Chinese Academy of Sciences, Harbin 150081, China;3.Heilongjiang Seed Industry Technical Service Center, Harbin 150008 |
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| Abstract: | 【Objective】 CRISPR/Cas9-mediated genome editing has become an important way for molecular breeding in rice. To promote rice breeding, the non-fragrant japonica rice variety Longjing11 was used as the test material. This study aims to edit GS3, GS9 and Badh2 genes for obtaining valuable and stable long-grain fragrant rice materials. 【Method】 The target genes GS3, GS9 and BADH2 were selected to construct the vector pYLCRISPR/ Cas9-GS3/ GS9/Badh2-gRNA by using CRISPR/Cas9 gene editing technology. The Agrobacterium-mediated transformation was used to transform Longjing11. Specific mutations were introduced into the GS3, GS9 and Badh2 genes in Longjing 11. 【Result】 The grain length of the transgenic free homozygote gs3/gs9/badh2 increased by 26.43% to 27.01%, yield per plant by 10.82% to 12.11%, 1000-grain weight by 18.34% to 41.36%, rice became fragrant as compared with wild type of Longjing 11. This study efficiently transformed round-grain rice into long-grain fragrant rice. 【Conclusion】The homozygous mutant lines featured by stable inheritance and long-grain fragrant quality were obtained by using CRISPR/Cas9 system. This study provides a convenient and effective way of combining multiple quality traits together, which could significantly accelerate breeding process from a breeding perspective. |
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| Keywords: | rice grain shape quality breeding fragrant rice CRISPR/Cas9 |
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