Differential Proteomic Analysis between Sporulated Oocyst and Unsporulated Oocyst of Cystoisospora suis |
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| Authors: | YANG Shoushen SUN Xiaoshuang QIU Yunfei YANG Fei TANG Jie LIAN Wenjian HUANG Cuiqin CHIU Hungchuan |
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| Institution: | 1. Longyan University&Fujian Provincial Key Laboratory for the Prevention and Control of Animal Infectious Diseases and Biotechnology, Longyan 364012, China;2. College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China;3. Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China;4. College of Animal Science, Tibetan Agricultural and Animal Husbandry College, Linzhi 860000, China |
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| Abstract: | The differentially expressed proteins between sporulated oocyst and unsporulated oocyst of Cystoisospora suis strains were screened and compared. In this study, the C. suis infection model of piglets were established and a quantitative proteomic analysis between sporulated oocyst and unsporulated oocyst of C. suis were conducted by iTRAQ labeling and LC-MS/MS. Part of the iTRAQ results were validated by qRT-PCR. Total 174 proteins were identified, among which 40 proteins were differentially expressed, 38 proteins were upregulated and 2 proteins were downregulated. The differentially expressed proteins were mainly involved in metabolism, antibiotics biosynthesis, secondary metabolites biosynthesis and glycolysis/gluconeogenesis pathways. The results of this study provide reference for elucidating the developmental mechanism of C. suis sporulated oocyst and facilitated discovering a new biomarker for C. suis. |
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| Keywords: | Cystoisospora suis sporulated oocyst unsporulated oocyst iTRAQ |
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