| 红麻海藻糖生物合成关键酶基因HcTPPJ的克隆及响应逆境的表达分析 |
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| 基金项目： | China Agriculture Research System(CARS-19-E07);Agricultural Science and Technology Innovation Program at the Chinese Academy of Agricultural Science(ASTIP-IBFC03);Hunan Education Department Project(18C0737);Doctoral Research Start-up Project of Hunan University of Arts and Sciences(17BSQD13) |
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| 摘 要： |
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| 收稿时间： | 2020-01-10 |
Cloning of the key enzyme gene HcTPPJ in trehalose biosynthesis of kenaf and its expression in response to abiotic stress in kenaf |
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| Authors: | Hui LI De-Fang LI Yong DENG Gen PAN An-Guo CHEN Li-Ning ZHAO Hui-Juan TANG |
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| Institution: | 1.College of Life and Environment Science, Hunan University of Arts and Science, Changde 415000, Hunan, China;2.Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, Hunan, China |
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| Abstract: | Trehalose biosynthesis key enzyme gene TPP plays an important role in plant response to various abiotic stresses. In this study, in order to clarify the role of TPP gene in response to abiotic stress in kenaf, a specific primer was designed according to the sequence of CL541.contig2unigene, and the full-length cDNA sequence of TPP gene was obtained by PCR. Bioinformatics analysis showed that TPP had an open reading frame (ORF) length of 1128 bp, and encoded a protein containing 375 amino acids. The results of amino acid sequence consistency indicated that the agreement between the amino acid sequence of protein and that of TPPJ from other species was 71.18%, so the gene named as HcTPPJ. HcTPPJ was expressed in roots, stems and leaves. Under salt or drought stress, HcTPPJ was up-regulated significantly with the extension of stress treatment, indicating that the gene was involved in the response process of salt or drought stress in kenaf. Under the same conditions, HcNCED3 and HcAOC was significantly up-regulated, while the change of HcTPPJ expression was not obvious under ABA stress; HcTPPJ was significantly down-regulated, under the stress of MeJA for six hours. Therefore it was speculated that the HcTPPJ gene expression may be not regulated by the signal molecule of ABA, but negatively regulated by methyl jasmonate signal molecule. This study will lay a solid foundation for further elucidating the role of the gene in response to salt and drought stress in kenaf. |
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| Keywords: | kenaf salt stress trehalose-6-phosphate phosphatase ABA |
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