| 甘薯IbCAF1基因的克隆及耐盐性、抗旱性鉴定 |
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| 基金项目： | National Natural Science Foundation of China(31872878);National Key Research and Development Program of China(2018YFD1000700);National Key Research and Development Program of China(2018YFD1000704);China Agriculture Research System(CARS-10) |
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| 摘 要： |
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| 收稿时间： | 2020-02-27 |
Cloning of IbCAF1 and identification on tolerance to salt and drought stress in sweetpotato |
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| Authors: | Shan-Bin CHEN Si-Fan SUN Nan NIE Bing DU Shao-Zhen HE Qing-Chang LIU Hong ZHAI |
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| Institution: | Key Laboratory of Sweetpotato Biology and Biotechnology, Ministry of Agriculture and Rural Affairs / Laboratory of Crop Heterosis and Utilization, Ministry of Education / Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, China |
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| Abstract: | CAF1 (CCR4-associated factor 1) gene plays an important role in plant development and disease resistance. In this study, the IbCAF1 gene of sweetpotato was cloned according to the EST sequence. The ORF of IbCAF1 was 846 bp, encoding 281 amino acids, with a molecular weight of 32.13 kD and an isoelectric point of 4.83. The results of amino acid sequence alignment and phylogenetic tree analysis showed that IbCAF1 had higher homology with ItlCAF1, a homologous protein of Ipomoea triloba (2x), and the homology was 96.8%. IbCAF1 gene was induced and expressed by NaCl, PEG, ABA, and H2O2. The IbCAF1 gene was transferred into tobacco by Agrobacterium tumefaciens mediated transformation. The overexpression of IbCAF1 gene significantly improved the salt and drought tolerance of transgenic tobacco plants. After 200 mmol L -1NaCl and 10% PEG-6000 treatments, the transgenic tobacco plants showed significant upregulation of the genes involved in ROS scavenging system and proline biosynthesis related genes, significant increase of SOD activity, POD activity and proline content and significant decrease of H2O2 and malondialdehyde contents. These results demonstrate that the IbCAF1 gene could improve salt and drought tolerance in transgenic tobacco. This study will lay a foundation on salt and drought tolerance gene engineering of IbCAF1 gene in sweetpotato for the following research. |
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| Keywords: | sweetpotato IbCAF1 transgenic tobacco salt tolerance drought tolerance |
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