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Mapping quantitative trait loci controlling pre-harvest sprouting resistance in a red × white seeded spring wheat cross
Authors:B Fofana  D G Humphreys  G Rasul  S Cloutier  A Brûlé-Babel  S Woods  O M Lukow  D J Somers
Institution:(1) Cereal Research Centre, Agriculture and Agri-Food Canada, 195 Dafoe Rd, Winnipeg, MB, Canada, R3T 2M9;(2) Department of Plant Sciences, University of Manitoba, Winnipeg, MB, Canada, R3T 2N2
Abstract:Hard white wheat (Triticum aestivum L.) is a value-added product because of its processing advantages over red wheat; however, white wheat tends to be more susceptible to pre-harvest sprouting (PHS). To identify quantitative trait loci (QTLs) associated with PHS tolerance, we developed a doubled haploid (DH) mapping population from the cross AC Domain (red seeded) × White-RL4137 (white seeded). A genetic map was constructed using microsatellite markers located on chromosome groups 3, 4, 5 and 6. A population of 174 DH lines was characterized for important aspects of PHS including sprouting index, germination index, Hagberg falling number and seed coat colour. A total of 11 QTLs were identified on group 3 chromosomes and on chromosome 5D. Seven QTLs associated with the PHS traits were found to be co-incident with seed coat colour on chromosomes 3A, 3B and 3D. The 5D PHS QTL was notable because it is independent of seed coat colour.
Keywords:Breeding  Mapping  Microsatellite markers  Pre-harvest sprouting  QTLs  White wheat
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