| 中华芦荟的离体快繁技术研究 |
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| 引用本文： | 宛淑艳,袁迎,陈春莲.中华芦荟的离体快繁技术研究[J].安徽农业科学,2009,37(10):4382-4383. |
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| 作者姓名： | 宛淑艳 袁迎 陈春莲 |
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| 作者单位： | 湛江师范学院生命科学与技术学院,广东湛江,524048;湛江师范学院生命科学与技术学院,广东湛江,524048;湛江师范学院生命科学与技术学院,广东湛江,524048 |
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| 基金项目： | 湛江师范学院校内博士专项(ZL0604) |
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| 摘 要： | 目的]探索芦荟的离体快繁技术,为芦荟的组织培养提供试验依据。方法]以盆栽中华芦荟的嫩茎尖为外植体,MS为基本培养基,不同的增殖阶段添加不同的植物激素进行离体快繁试验。结果]培养30 d后,3瓶被污染,4瓶不长愈伤组织,13瓶在芽基部产生圆球状浅黄色的愈伤组织。在诱导芽分化约20 d后,2瓶被污染,11瓶开始芽点分化。3.00 mg/L 6-BA的处理效果最佳。诱导生根11 d后,1瓶被污染,2瓶出现叶子干焦的现象,17瓶长势良好。中华芦荟愈伤组织诱导的适宜培养基为MS＋6-BA 2.50 mg/L＋NAA0.15 mg/L,诱导率为60%;丛生芽分化及继代的适宜培养基为MS＋6-BA 3.00 mg/L＋NAA 0.10 mg/L,诱导率为85%;生根的适宜培养基为MS＋6-BA 0.30～0.50 mg/L＋IBA 0.20 mg/L＋活性炭0.5%。结论]该研究为芦荟的快繁提供了初步的理论依据。
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| 关 键 词： | 中华芦荟 茎尖 离体快繁技术 |
Research in the Rapid Propagation in vitro of Aloe vera var. chinensis |
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| WAN Shu-yan et al.Research in the Rapid Propagation in vitro of Aloe vera var. chinensis[J].Journal of Anhui Agricultural Sciences,2009,37(10):4382-4383. |
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| Authors: | WAN Shu-yan |
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| Institution: | WAN Shu-yan et al(College of Life Science , Technology,Zhanjiang Normal University,Zhanjiang,Guangdong 524048) |
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| Abstract: | Objective] The research aimed to explore the rapid propagation in vitro of Aloe vera and provide experimental basis for its tissue culture.Method] With tender shoot tips of potted Aloe vera var.chinensis as explants,MS as basic medium,different phytohormones were added in different multiplicative stages for the experiment on its rapid propagation in vitro.Result] After culture for 30 d,3 flasks were polluted,there was no callus grown in 4 flasks and there were spherical and light yellow calli generated a... |
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| Keywords: | Aloe vera var chinensis Shoot tip Rapid propagation in vitro |
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