| 毛竹快速拔节过程节间组织cDNA文库的构建与分析 |
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| 引用本文： | 郑丽娜,周明兵.毛竹快速拔节过程节间组织cDNA文库的构建与分析[J].福建林业科技,2012,39(3):19-23. |
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| 作者姓名： | 郑丽娜 周明兵 |
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| 作者单位： | 亚热带森林培育国家重点实验室培育基地,浙江农林大学,浙江临安311300 |
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| 基金项目： | 国家自然科学基金项目(31070590);浙江农林大学教改项目(YB1106、YB0904);浙江农林大学“分子生物学精品课程”资助 |
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| 摘 要： | 以同一棵毛竹笋上快速伸长生长的节间组织和基本停止伸长生长的节间组织为材料,分别提取总RNA,纯化成mR-NA后合成cDNA双链;以Uni-ZAP XR vector为载体,构建了2个cDNA文库。快速伸长生长的、停止伸长生长的节间组织cDNA文库的滴度分别为9.7×109、8.4×109pfu.mL-1,含插入片段的频率均达到99%以上,插入片段的大小均在500～3000 bp。cDNA文库的成功构建为探究毛竹快速生长的分子机理奠定了基础。
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| 关 键 词： | 毛竹 节间组织 cDNA文库 |
Construction and Analysis of cDNA Libraries of the Rapidly-Elongating Internodes of Phyllostachys pupescens |
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| ZHENG Li-na
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ZHOU Ming-bing.Construction and Analysis of cDNA Libraries of the Rapidly-Elongating Internodes of Phyllostachys pupescens[J].Journal of Fujian Forestry Science and Technology,2012,39(3):19-23. |
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| Authors: | ZHENG Li-na ZHOU Ming-bing |
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| Institution: | (The Nurturing Station for the State Key Laboratory of Subtropical Xilviculture, Zhejiang A&F University,Lin’an 311300,Zhejiang,China) |
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| Abstract: | The total RNA was extracted respectively from rapidly-elongating internodes and static internodes of Phyllostachys pupescens.After mRNA was purified,2 cDNA libraries were constructed with mRNA using Uni-ZAP XR vector.The cDNA libraries of rapidly-elongating internodes and static internodes consisted of 9.7×109pfu·mL-1 and 8.4×109pfu·mL-1 respectively.Both of cDNA libraries contained over 99% recombinant phages,in which inserts ranged between 500bp and 3000bp in sizes.The results indicated that the cDNA libraries successful construction was the base for studying molecular mechanism of rapid internodal elongation in P.pupescens. |
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| Keywords: | Phyllostachys pupescens internode cDNA library |
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