| 上西早生柿ACC氧化酶的转基因研究 |
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| 引用本文： | 刘艺,马俊莲,张子德,唐霞,宋春丽.上西早生柿ACC氧化酶的转基因研究[J].河北农业大学学报,2009,32(2). |
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| 作者姓名： | 刘艺 马俊莲 张子德 唐霞 宋春丽 |
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| 作者单位： | 1. 河北农业大学,食品科技学院,河北,保定,071001
2. 河北农业大学,中兽医学院,河北,定州,073000 |
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| 基金项目： | 河北省国际科技合作攻关项目 |
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| 摘 要： | 为了得到耐贮藏的转基因柿,以上西早生柿叶片为外植体,通过农杆菌介导法,建立了ACC氧化酶的RNAi遗传转化体系。研究了预培养时间、农杆菌菌液浓度、侵染时间、乙酰丁香酮(AS)浓度和共培养时间等因素对基因转化的影响。结果表明:适宜的壮观霉素浓度为30 mg/L,预培养时间为3 d,用OD600值为0.3的菌液侵染10 min、AS浓度100 mg/L、共培养4 d有利于提高转化频率。经PCR分子检测与GUS组织化学染色,初步确定ACC氧化酶基因已转入上西早生柿组培苗中。
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| 关 键 词： | 上西早生柿 根癌农杆菌 ACC氧化酶基因(ACO) 遗传转化 |
Study on transformation of Uenishiwase Diospyros kaki Thunb with ACC oxidase gene |
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| LIU Yi,MA Jun-lian,ZHANG Zi-de,TANG Xia,SONG Chun-li.Study on transformation of Uenishiwase Diospyros kaki Thunb with ACC oxidase gene[J].Journal of Agricultural University of Hebei,2009,32(2). |
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| Authors: | LIU Yi MA Jun-lian ZHANG Zi-de TANG Xia SONG Chun-li |
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| Institution: | 1.College of Food Science and Technology;Agricultural University of Hebei;Baoding 071001;China;2.College of Chinese Veterinary;Dingzhou 073000;China |
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| Abstract: | In order to get transgenic Japanese Persimmon,using young leaves of Uenishiwase as explant,ACC oxidase gene was transferred into Uenishiwase,mediated by Agrobacterium tumefaciens.The pre-cultured time,the concentration of A.tumefaciens,the infection time,the concentration of AS and the co-cultured time were studied.Together studied was the genetic transformation systems.The results showed that the available concentration of Spectinomycin was 10 mg/L,the best pre-cultured time was 3 d,the fittest OD600 of en... |
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| Keywords: | Uenishiwase Agrobacterium tumefaciens ACC oxidase gene(ACO) genetic transformation |
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