| 细菌发酵产L-乳酸优良菌株的选育 |
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| 引用本文： | 张婵婵,潘丽军,赵丹丹.细菌发酵产L-乳酸优良菌株的选育[J].农产品加工．学刊,2006(10):23-26,43. |
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| 作者姓名： | 张婵婵 潘丽军 赵丹丹 |
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| 作者单位： | 合肥工业大学,生物与食品工程学院,合肥,230009 |
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| 基金项目： | 高等学校博士学科点专项科研项目 |
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| 摘 要： | 对干酪乳杆菌CICC6002进行紫外诱变和硫酸二乙酯—超声波复合诱变处理,将诱变所得菌株进行耐酸和耐高糖驯化和选育,筛选得到一株高产L-乳酸的正向突变NT-7,在以菜籽饼粕水解液为氮源的培养基中发酵72h,L-乳酸产量88g/L,较出发菌株提高了41.94%。
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| 关 键 词： | 干酪乳杆菌 L-乳酸 诱变 驯化 发酵 |
| 文章编号： | 1671-9646（2006）10-0004-04 |
| 收稿时间： | 2006-08-22 |
| 修稿时间： | 2006年8月22日 |
Screening of L-lactic Acid High-producing Bacteria |
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| Zhang Chanchan,Pan Lijun,Zhao Dandan.Screening of L-lactic Acid High-producing Bacteria[J].Nongchanpin Jlagong.Xuekan,2006(10):23-26,43. |
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| Authors: | Zhang Chanchan Pan Lijun Zhao Dandan |
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| Abstract: | Ultraviolet light and DES- ultrasound mutagenesis were applied to Lactobacillus casei CICC6002. The high-producing strain NT-7 was finally selected after being acculturated by MRS acid media and MRS high-sugar slab. After fermentation for 72 hours in the fermentation media by using rapeseed meal hydrolyte as nitrogen source, the mutant strain NT-7 gave an L-lactic acid output of 88 g/L, 41.94% higher than the parent strain. |
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| Keywords: | Lactobacillus casei L-lactic acid mutation acculturation fermentation |
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