| 中草药犁头草的体外抑菌作用研究 |
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| 引用本文： | 赵锦慧,葛红莲,赖颖,郭婕.中草药犁头草的体外抑菌作用研究[J].河南农业科学,2012,41(2):146-148. |
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| 作者姓名： | 赵锦慧 葛红莲 赖颖 郭婕 |
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| 作者单位： | 周口师范学院生命科学系,河南周口,466000 |
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| 基金项目： | 河南省教育厅自然科学基金 |
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| 摘 要： | 为测定中草药犁头草的体外抑菌作用,利用平板菌落计数法筛选103 cfu/mL的大肠杆菌和金黄色葡萄球菌菌液,并将其涂布到细菌培养基与不同质量浓度中草药犁头草混合的药液培养基上,测定犁头草对大肠杆菌和金黄色葡萄球菌的抑菌率和最小抑菌浓度。结果显示,犁头草对大肠杆菌和金黄色葡萄球菌的最小抑菌质量浓度分别为250mg/mL和62.5mg/mL;在药液质量浓度不小于最小抑菌质量浓度时,犁头草对以上2种细菌的抑菌率均为100%。由此看出,犁头草对大肠杆菌和金黄色葡萄球菌均有明显的体外抑制作用,且对金黄色葡萄球菌的抑制效果优于大肠杆菌。
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| 关 键 词： | 中草药 犁头草 大肠杆菌 金黄色葡萄球菌 抑菌率 最小抑菌浓度 |
Study on the in vitro Antibacterial Action of Chinese Herbal Medicine Viola japonica Langsd. |
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| ZHAO Jin-hui
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GE Hong-lian
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LAI Ying
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GUO Jie.Study on the in vitro Antibacterial Action of Chinese Herbal Medicine Viola japonica Langsd.[J].Journal of Henan Agricultural Sciences,2012,41(2):146-148. |
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| Authors: | ZHAO Jin-hui GE Hong-lian LAI Ying GUO Jie |
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| Institution: | (Department of Life Science,Zhoukou Normal University,Zhoukou 466000,China) |
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| Abstract: | The bacteria liquid with 103 cfu/mL was prepared by flat calculating colony,which was smeared on the mixture of normal bacteria culture medium and Viola japonica Langsd.with di-fferent concentration and the inhibition effects of Viola japonica Langsd.E.coli and Staphylococcus aureus were evaluated by the inhibitory rate and MIC.The results showed that Viola japonica Langsd.Represent obvious in vitro inhibitory action to E.coli and Staphylococcus aureus.The inhibitory effect to Staphylococcus aureus was superior to E.coli.The minimal Inhibitory concentration(MIC) of Viola japonica Langsd.to E.coli and Staphylococcus aureus was 250 mg/mL and 62.5 mg/mL,respectively.The inhibitory rate was 100% when the concentration of liquor was equal or greater than that of the MIC. |
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| Keywords: | Chinese herbal medicine Viola japonica Langsd E coli Staphylococcus aureus inhibitory rate minimal inhibitory concentration(MIC) |
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